| Study in vitro of populating autogenous Schwann cells into chemical extracted allogenous nerve |
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| 引用本文： | 王建云,刘小林,朱家恺,李文萍,向剑平. Study in vitro of populating autogenous Schwann cells into chemical extracted allogenous nerve[J]. 中华创伤杂志(英文版), 2002, 5(6): 326-328 |
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| 作者姓名： | 王建云 刘小林 朱家恺 李文萍 向剑平 |
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| 作者单位： | DepartmentoifMicrosurgery,FirstAffiliatedHospital,Sunyat－SenUniversity,Guangzhou510089,China |
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| 基金项目： | 广东省自然科学基金，National Natural Sciences |
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| 摘 要： | Objective:To look for an ideal substance to repair large gap of nerve difect after injury by culture of Schwann cells(Scs)and preparation of acellular allogenous nerve grafts(ANG) with chemical extraction.Methods:The double adhesion culture and Arab-c to prohibit the fibroblast growth were used to achieve high-purified Scs.Triton-x-100 and sodium deoxycholate were used to achieve ANG.Finally the Scs were microinjected into the acellural nerve grafts and cultured in vitro.The consequence was analysed.Results:High purified Scs and ANG were acquired, which could integrate each other well.Scs could survive and transfer to aline in vitro.Conclusions:Populating Scs into chemical extracted ANG may be an ideal substance to repair the large gap of nerve defect after injury.
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| 关 键 词： | 自体雪旺氏细胞 化学提取异体神经 体外培养 神经移植 |
Study in vitro of populating autogenous Schwann cells into chemical extracted allogenous nerve |
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| Jianyun Wang,Xiaolin Liu,Jiakai Zhu,Wenping Li,Jianping Xiang. Study in vitro of populating autogenous Schwann cells into chemical extracted allogenous nerve[J]. Chinese journal of traumatology, 2002, 5(6): 326-328 |
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| Authors: | Jianyun Wang Xiaolin Liu Jiakai Zhu Wenping Li Jianping Xiang |
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| Affiliation: | Department of Microsurgery, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510089, China |
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| Abstract: | OBJECTIVE: To look for an ideal substance to repair large gap of nerve defect after injury by culture of Schwann cells (Scs) and preparation of acellular allogenous nerve grafts (ANG) with chemical extraction. METHODS: The double adhesion culture and Arab-c to prohibit the fibroblast growth were used to achieve high-purified Scs. Triton-x-100 and sodium deoxycholate were used to achieve ANG. Finally the Scs were microinjected into the acellural nerve grafts and cultured in vitro. The consequence was analysed. RESULTS: High purified Scs and ANG were acquired, which could integrate each other well. Scs could survive and transfer to aline in vitro. CONCLUSIONS: Populating Scs into chemical extracted ANG may be an ideal substance to repair the large gap of nerve defect after injury. |
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| Keywords: | Schwann cells Nerves tissue Transplants |
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