| 结核分枝杆菌ESAT-6与CFP-10蛋白间的相互作用 |
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| 引用本文： | 李岩,鲍朗,张会东,王晓樱,朱海龙,李娅莎.结核分枝杆菌ESAT-6与CFP-10蛋白间的相互作用[J].四川大学学报(医学版),2006,37(3):349-352. |
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| 作者姓名： | 李岩 鲍朗 张会东 王晓樱 朱海龙 李娅莎 |
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| 作者单位： | 四川大学华西基础医学与法医学院,感染免疫研究室,成都,610041;四川大学华西基础医学与法医学院,感染免疫研究室,成都,610041;四川大学华西基础医学与法医学院,感染免疫研究室,成都,610041;四川大学华西基础医学与法医学院,感染免疫研究室,成都,610041;四川大学华西基础医学与法医学院,感染免疫研究室,成都,610041;四川大学华西基础医学与法医学院,感染免疫研究室,成都,610041 |
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| 摘 要： | 目的观察结核分枝杆菌ESAT-6与CFP-10蛋白间的相互作用。方法采用PCR技术克隆结核分枝杆菌ESAT-6与CFP-10基因，构建酵母双杂交载体质粒pGBKT7-ESAT-6和pGADT7-CFP-10，经酶切分析和DNA测序证实连接片断的正确性后，采用醋酸锂法顺序转染酵母菌AH109。结果共转染pGBKT7-ESAT-6和pGADT7-CFP-10的酵母菌AH109可以在SD／-Ade／-His／-Leu／-Trp培养基上生长，β-半乳糖苷酶活性实验阳性。结论结核分枝杆菌ESAT-6与CFP-10蛋白可以相互作用。
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| 关 键 词： | ESAT-6 CFP-10 酵母双杂交 蛋白间相互作用 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-12-02 |
Protein-protein Interaction Between ESAT-6 and CFP-10 of Mycobacterium Tuberculosis |
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| LI Yan,BAO Lang,ZHANG Hui-dong,WANG Xiao-ying,ZHU Hai-long,LI Ya-sha.Protein-protein Interaction Between ESAT-6 and CFP-10 of Mycobacterium Tuberculosis[J].Journal of West China University of Medical Sciences,2006,37(3):349-352. |
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| Authors: | LI Yan BAO Lang ZHANG Hui-dong WANG Xiao-ying ZHU Hai-long LI Ya-sha |
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| Institution: | Research Unit of Infection and Immunity, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. |
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| Abstract: | Objective To investigate the protein-protein interaction between ESAT-6 and CFP-10 of mycobacterium tuberculosis. Methods ESAT-6 gene and CFP-10 gene were amplified from mycobacterium tuberculosis genome DNA. The ESAT-6 gene was subcloned into pGBKT7 and the CFP-10 gene was subcloned into pGADT7-Rec. After being verified with restriction endonuclease digestion and DNA sequencing, the recombinant vectors were transformed into yeast cell AH109 by lithium acetate method. Results The yeast cells co-transformed with pGBKT7-ESAT-6 and pGADT7-CFP-10 grew on SD/-Ade/-His/-Leu/-Trp plates, and galactosidase activity assays showed positive results. Conclusion ESAT-6 and CFP-10 protein could interact with each other in yeast cells. |
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| Keywords: | ESAT-6 CFP-10 |
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