常温肝缺血再灌注肝血窦内皮细胞损伤

目的 探讨肝脏常温 因再灌注稆肝血窦内皮细胞的结构变化及其在再灌注损伤中的作用。方法 将４４只大鼠在常温下分别阻断人肝血流２０、４０、６０和９０ｍｉｎ,然后再开放血流２ｈ,制备成缺血再灌注模型,对肝血窦内皮细胞进行扫描和透射电镜观察,正常对照组大鼠５只。结果 肝血流阻断２０、４０ｍｉｎ时内皮细胞受损;血流开放后２ｈ内皮变化可恢复。肝血流肝断６０、９０ｍｉｎ后内皮细胞出现损伤,使部分内皮缺损,上直接

Liver sinusoidal endothelial cell damage during normothermic ischemia and reperfusion of rat

Objective To observe the damage to liver sinusoidal endothelial cells during normothermic ischemia and reperfusion.Methods The afferent vessels of rat liver were clamped for 20,40,60 and 90 min respectively. The blood flow to the liver then was restored by removing the clamp. Ultrastructural changes in the sinusoidal endothelial cells in various periods were observed. Results After 20 or 40 min ischemia, fenestrations of the endothelial cells were enlarged and became mesh like, but reperfusion for 120 min, led to recovery. After 60 or 90 min ischemia, tho sieve plates and cytoplasmic processes of the endothelial cells were destroyed and the perikaryon tended to be desquaments. Reperfusion for 120 min led to no recovery, but resulted in the aggravation of injury and irreversible damage. The liver sinusoids were filled with numerous blood cells and blebs derived from hepatocytes, resulting in circulatory disturbance.]ConclusionsWarm ischemia results in severe endothelial cell destruction. Endothelial cell damages may have unfavorable effects on the microcirculation of the reperfusional liver after prolonged ischemia.