| 食管黏膜树突状细胞表型分析 |
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| 作者姓名： | Wang CL Ning HB Wang YH Zhang LF Li JC |
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| 作者单位： | 郑州大学第一附属医院药剂科;郑州大学第一附属医院消化内科 |
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| 基金项目： | 河南省教育厅自然科学研究资助计划项目(2010A320031) |
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| 摘 要： | 目的:研究食管黏膜树突状细胞(DCs)表型特点。方法:内镜下收集健康人食管黏膜标本,Ficoll-Hypaque密度梯度离心法分离食管黏膜标本中的单个核细胞,采用磁珠分选技术分离DCs,流式细胞仪分析食管黏膜DCs表型。结果:健康人食管黏膜含3类单个核细胞:HLA-DRhigh/CD13low,HLA-DRmed/CD13+和HLA-DR-/CD13+;HLA-DRhigh/CD13low细胞表达不同DCs亚群表面标志,是食管黏膜DCs;健康人食管黏膜DCsCD80、CD83、CD86表达水平低,是不成熟DCs。结论:成功分离了食管黏膜DCs,证实HLA-DRhigh/CD13low细胞是食管黏膜DCs。
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| 关 键 词： | 食管黏膜 树突状细胞 |
Isolation and characterization of dendritic cells from normal human esophageal mucosa |
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| Wang CL,Ning HB,Wang YH,Zhang LF,Li JC.Isolation and characterization of dendritic cells from normal human esophageal mucosa[J].Journal of Cellular and Molecular Immunology,2012,28(7):733-4, 737. |
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| Authors: | Wang Cun-liang Ning Han-bing Wang Yan-hong Zhang Lian-feng Li Ji-chang |
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| Institution: | Department of Pharmacy, First Affiliated Hospital, Zhengzhou University, China. |
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| Abstract: | AIM: To investigate the characteristics of dendritic cells(DCs) from normal human esophageal mucosa.METHODS: We collected normal esophageal mucosa samples from healthy individuals using endoscope and isolated mononuclear cells from the samples using Ficoll-Hypaque density gradient centrifugation.DCs were further separated by magnetic-activated cell sorting(MACS) to analyze DCs subsets by flow cytometry.RESULTS: Three mononuclear cell populations were detected in normal human esophageal mucosa: HLA-DRhigh/CD13low,HLA-DRmed/CD13+ and HLA-DR-/CD13+.HLA-DRhigh/CD13low cells expressed the DCs-associated molecules and were referred to as esophageal mucosal DCs.The DCs were immature since they expressed low levels of CD80,CD83 and CD86.CONCLUSION: HLA-DRhigh/CD13low cells isolated from the normal human esophageal mucosa were proved to be DCs.DCs can be successfully isolated from esophageal mucosa. |
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| Keywords: | esophageal mucosa dentritic cells |
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