| 急性非淋巴细胞白血病中MGMT与p53基因突变的研究 |
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| 引用本文： | 李晓雯,郑红,连建华,齐华,游文风,宋国英,陈玉清.急性非淋巴细胞白血病中MGMT与p53基因突变的研究[J].河北医科大学学报,1999,20(5):272-273. |
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| 作者姓名： | 李晓雯 郑红 连建华 齐华 游文风 宋国英 陈玉清 |
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| 作者单位： | 1. 河南医科大学细胞生物学与医学遗传学教研室,郑州,450052
2. 河南省人民医院血液病研究所 |
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| 摘 要： | 目的 探讨肿瘤抑制基因ｐ５３、ＭＧＭＴ在急性非淋巴细胞白血病（ＡＮＬＬ）中的改变。方法 用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）技术对３１例ＡＮＬＬ患者、２２例正常人ｐ５３基因第５、６、７、８外显子和ＭＧＭＴ基因第１、４外显子进行突变分析。结果 ３１例ＡＮＬＬ中５例存在ｐ５３基因突变、４例存在ＭＧＭＴ基因突变，其中２例ｐ５３与ＭＧＭＴ同时发生突变。结论 ｐ５３基因突变在白血病发生中起一定
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| 关 键 词： | 白血病 非淋巴细胞 急性 基因 p53 DNA突变分析 聚合酶链反应 |
| 修稿时间： | 1999-06-07 |
STUDY OF MUTATION OF MGMT AND p53 IN ACUTE NON-LYMPHOCYTIC LEUKEMIA |
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| Li Xiaowen,Zheng Hong,Lian Jianhua,Qi Hua,You Wenfeng,Song Guoying,Chen Yuqing.STUDY OF MUTATION OF MGMT AND p53 IN ACUTE NON-LYMPHOCYTIC LEUKEMIA[J].Journal of Hebei Medical University,1999,20(5):272-273. |
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| Authors: | Li Xiaowen Zheng Hong Lian Jianhua Qi Hua You Wenfeng Song Guoying Chen Yuqing |
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| Institution: | Zhengzhou 450052 |
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| Abstract: | ObjectiveTo investigate the incidence of p 53 and MGMT gene alteration in acute non lymphocytic leukemia(ANLL).MethodsUsing polymerase chain reaction single stand conformation polymorphism(PCR-SSCP) technique,31 cases of acute non lymphocytic leukemia(ANLL) and 22 normal controls were studied for mutation in p53 gene exons 5,6,7,8 and MGMT gene exons1,4.ResultsIn 31 cases of ANLL,4 cases showed MGMT gene mutation,5 cases p53 gene mutation and 2 cases both p53 and MGMT mutation.Conclusionp53 gene mutation may play an important role in the pathogenesis of acute leukemia,while the role of MGMT gene mutation remains to be elucidated. |
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| Keywords: | leukemia nonlymplyocytic acute genes p53 DNA mutational analysis polymerase chain reaction |
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