| 黎芦碱对分离的大鼠神经细胞内游离钙的影响 |
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| 引用本文： | 卢步峰,鲁友明,黄森. 黎芦碱对分离的大鼠神经细胞内游离钙的影响[J]. 中国药理学通报, 1994, 0(3) |
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| 作者姓名： | 卢步峰 鲁友明 黄森 |
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| 作者单位： | 镇江医学院生物化学研究室，海军神经生物学研究中心分子药理学研究室 |
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| 摘 要： | 用Ｆｕｒａ－２双波长荧光法测得分离的大鼠神经细胞静息胞内游离钙浓度（［Ｃａ２＋］ｉ）为１１９．４±９．１ｎｍｏｌ·Ｌ－１（ｎ＝８）．藜芦碱（１０－４ｍｏｌ·Ｌ－１）可显著促进Ｃａ２＋内流而使［Ｃａ２＋］ｉ升高（Ｐ＜０．０１，ｎ＝８）；尼莫地平对静息［Ｃａ２＋］ｉ无影响．但能浓度依赖性地对抗藜芦碱所致［Ｃａ２＋］ｉ的升高。提示藜芦碱诱发的［Ｃａ２＋］ｉ升高由存在于神经细胞膜上的Ｌ型电压依赖性钙通道介导；尼莫地平可用以间接地保护神经细胞免受藜芦碱的兴奋毒性。
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| 关 键 词： | 大鼠，神经细胞，细胞内游离钙，藜芦碱，尼莫地平 |
Effects of veratridine on intracellular free Ca2十concentration in dissociated rat neurons |
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| LU Bu-Feng,LU You-Ming, HUANG Yi-Sen. Effects of veratridine on intracellular free Ca2十concentration in dissociated rat neurons[J]. Chinese Pharmacological Bulletin, 1994, 0(3) |
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| Authors: | LU Bu-Feng LU You-Ming HUANG Yi-Sen |
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| Abstract: | The intracellular free Ca2+concentration([Ca2十]1) was measured in freshly dissociated neurons prepared from SpragueDawley rats using the fluorescent Ca2+indicatorFura-2/AM. The resting[Ca2+]1 of neurons inCa2+-containing solution was 119.4±9.1nmol· L-1(,n=8).Veratridine(a Na+ channel blocker,10-4 mol· L-1) markedlyevoked[Ca2+]1 of neurons(P<0.01,n=8)and this increase could be prevented or reversedby nimodipine known to block L-type voltagedePendent Ca2+channels.These results suggestthat the rise of [Ca2+]1 induced by veratridine isa L-type voltage-dependent Ca2+ channel-mediated response and nimodipine may indirectly prevent neurons from veratridine-induced neurotoxicity.Division of Molecular Pharmacology. |
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| Keywords: | rat neurons intracellularfree calcium veratidine nimodipine |
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