| 标本因素对荧光定量PCR检测HBV DNA的影响 |
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| 引用本文： | 郝维敏,刘杰,杨晓春.标本因素对荧光定量PCR检测HBV DNA的影响[J].蚌埠医学院学报,2011,36(3):295-297. |
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| 作者姓名： | 郝维敏 刘杰 杨晓春 |
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| 作者单位： | 安徽省宿州市立医院,检验科,234000;安徽省宿州市立医院,检验科,234000;安徽省宿州市立医院,检验科,234000 |
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| 摘 要： | 目的：探讨标本溶血、黄疸、脂血对荧光定量PCR检测乙型肝炎病毒（HBV）DNA的影响。方法：荧光定量PCR检测无溶血、无黄疸、非脂血血清和溶血、黄疸、高脂血清标本的HBV DNA。结果：溶血、黄疸、高脂血血清与无溶血、无黄疸、非脂血血清HBV DNA定量检测结果差异均无统计学意义（P〉0.05）。结论：标本溶血、黄疸、脂血对荧光定量PCR检测HBV DNA无干扰。
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| 关 键 词： | 黄疸 溶血 脂血 荧光定量聚合酶链反应 乙型肝炎病毒 脱氧核糖核酸 |
Sample factors affecting fluorescence quantitative PCR detection of HBVDNA |
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| HAO Wei-min,LIU Jie,YANG Xiao-chun.Sample factors affecting fluorescence quantitative PCR detection of HBVDNA[J].Journal of Bengbu Medical College,2011,36(3):295-297. |
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| Authors: | HAO Wei-min LIU Jie YANG Xiao-chun |
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| Institution: | HAO Wei-min,LIU Jie,YANG Xiao-chun(Department of Clinical Laboratory,Suzhou Municipal Hospital,Suzhou Anhui 234000,China) |
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| Abstract: | Objective:To explore the effects of abnormal sample characters,such as hemolysis,icterus and lipemia,on fluorescence quantitative PCR(FQ-PCR) detection of HBV DNA. Methods:Levels of serum HBV DNA in hyperlipemia with hemolysis,icterus and normal serum samples were detected by FQ-PCR. Results:There were no significant differences of HBV DNA level among the groups of hyperlipemia with hemolysis,icterus and the control(P>0.05). Conclusions:Hemolysis,icterus and lipemia of serum sample have no interference on F... |
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| Keywords: | hemolysis icterus lipemia fluorescence quantitative-polymerase chain reaction hepatitis B virus DNA |
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