破格救心汤对心力衰竭大鼠心肌细胞凋亡的影响

目的观察破格救心汤对心肌梗死后心力衰竭大鼠心肌细胞凋亡的影响。方法将Wistar大鼠手术建立心力衰竭模型后随机分成破格救心汤组(12只)、卡托普利组(12只)、模型组(12只),另选未经手术的Wistar大鼠12只作为对照组。破格救心汤组每日2次灌胃破格救心汤10 mL/kg;卡托普利组每日2次灌胃卡托普利液10 mL/kg,模型组和对照组每日2次灌胃饮用水10 mL/kg。每周称体质量1次,据此调整用药量。在实验第3周、第6周分别将大鼠心脏制成石蜡切片。采用原位末端标记(TUNEL)法检测心肌细胞凋亡,免疫组化法检测凋亡调控基因bax及bcl-2蛋白表达。结果破格救心汤组、卡托普利组动物心肌梗死边缘区凋亡阳性细胞较模型组明显减少,给药6周后,凋亡率更低。破格救心汤组中,bax蛋白表达平均光强度显著升高,bcl-2蛋白表达平均光强度明显降低。结论破格救心汤对心肌梗死后心力衰竭心肌细胞凋亡有改善作用,这可能与其对细胞凋亡调控基因的改变有关。

Influence of Poge saving heart decoction on myocardial cell''''s apoptosis of heart failure rat

Objective It is to observe the influence of Poge saving heart decoction(PSHD) on myocardial cell's apoptosis of heart failure rat after myocardial infarction.Methods The heart failure models were made with 36 Wistar rats and randomly divided into PSHD group(12 rats),Captopril group(12 rats) and model group(12 rats).Other 12 Wistar rats not operated on were chosen as control group.PSHD group were given PSHD 10 mL/kg twice a day by intragastric administration.Captopril group were given Captopril liquid 10 mL/kg twice a day by intragastric administration.Model group and control group were given drinking water 10 mL/kg twice a day by intragastric administration.Body mass was weighed once a week to adjust the dosage of drugs.In the third and sixth test week,the rat hearts were separately made into paraffin slices.Cardiac muscle cell apoptosis was examined with TUNEL method.The expressions of apoptosis regulative genes bax and bcl-2 protein were detected with immunohistochemical method.Results The positive apoptosis cells in the brim area of myocardial infarction in PSHD group and Captopril group reduced much more than those in model group,the apoptosis rate was lower especially six weeks after administer.The average light intensity of bax protein expression remarkably increased and that of Bcl-2 protein expression obviously decreased in PSHD group.Conclusion PSHD can improve the apoptosis of heart failure myocardial cells after cardiac infarction,which may be related with its changing the cell apoptosis regulative genes.