Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone |
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Authors: | Xu Hongmin Hu Furong Sado Yoshikazu Ninomiya Yoshifumi Borza Dorin-Bogdan Ungvari Zoltan Lagamma Edmund F Csiszar Anna Nedergaard Maiken Ballabh Praveen |
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Institution: | Department of Pediatrics, New York Medical College-Westchester Medical Center, Valhalla, New York 10595, USA. |
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Abstract: | Germinal matrix is selectively vulnerable to hemorrhage in premature infants, and use of prenatal betamethasone is associated with a lower occurrence of germinal matrix hemorrhage. Because the major components of extracellular matrix of the cerebral vasculature-laminin, fibronectin, collagen IV, and perlecan-provide structural stability to blood vessels, we examined whether the expression of these molecules was decreased in the germinal matrix and affected by betamethasone. In both human fetuses and premature infants, fibronectin was significantly lower in the germinal matrix than in the cortical mantle or white matter anlagen. Conversely, laminin alpha1 gene expression was greater in the human germinal matrix compared with the cortical mantle or white matter. Expression of alpha1- and alpha2(IV) collagen chains increased with advancing gestational age. Low-dose prenatal betamethasone treatment enhanced fibronectin level by 1.5-2-fold whereas a high dose reduced fibronectin expression by 2-fold in rabbit pups. Because fibronectin provides structural stability to the blood vessels, its reduced expression in the germinal matrix may contribute to the fragility of germinal matrix vasculature and the propensity to hemorrhage in premature neonates. |
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Keywords: | laminin fibronectin collagen IV perlecan GM cerebral cortex white matter germinal matrix hemorrhage intraventricular hemorrhage |
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