HPLC荧光检测法测定人血浆中的特拉唑嗪

目的采用HPLC荧光检测法测定人血浆中的特拉唑嗪。方法采用Apollo C18色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-50 mmol·L-1磷酸二氢钾缓冲液(22∶78),流速1.1 mL·min-1,柱温30℃,进样量10μL,荧光检测器激发波长为238 nm、发射波长为370 nm。以哌唑嗪为内标,血浆样本碱化后经乙醚-二氯甲烷(3∶2)提取后进样。结果特拉唑嗪的线性范围为0.5～125.0μg·L-1(r≥0.9983);日内、日间RSD均小于10%;平均提取回收率为88.0%±4.4%。结论所用方法简便、准确、灵敏,无杂质干扰,适用于临床上盐酸特拉唑嗪口服制剂的血药浓度监测及药动学研究。

Determination of terazosin in human plasma by HPLC with fluorescence detection

OBJECTIVE To establish an HPLC method with fluorescence detection for determination of terazosin in human plasma.METHODS The separation was carried out on an Apollo C18 column using the mobile phase of acetonitrile and 50 mmol·L-1 potassium dihydrogen phosphate buffer solution(22:78)at a flow rate of 1.1 mL·min-1.The column temperature was maintained at 30 ℃ and the injection volume was 10 μL.The excitation and emission wavelengths of the fluorescence detection were set at 237 nm and 370 nm,respectively.The analyte and prazosin(internal standard)were extracted with diethylether-dichlormethane(3:2) after alkalinized.RESULTS The linear range of terazosin in human plasma was 0.5-125.0 μg·L-1(r≥0.9983).The intra-and inter-day RSD were both less than 10%.The mean extraction recovery was 88.0%±4.4%.CONCLUSION The method is rapid,simple,sensitive and suitable for the clinical pharmacokinetic study of terazosin preparations.