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栀子药材的HPLC指纹图谱研究
引用本文:任淑娟,孙辉,丁野,李文莉. 栀子药材的HPLC指纹图谱研究[J]. 华西药学杂志, 2012, 27(3): 309-311
作者姓名:任淑娟  孙辉  丁野  李文莉
作者单位:任淑娟 (湖南中医药大学,湖南长沙410208) ; 孙辉 (湖南省食品药品检验研究院,湖南长沙410001) ; 丁野 (湖南省食品药品检验研究院,湖南长沙,410001) ; 李文莉 (湖南省食品药品检验研究院,湖南长沙,410001) ;
基金项目:国家“十一五”重大科技支撑计划“重大新药创制”课题
摘    要:目的建立栀子药材的HPLC特征指纹图谱,为其质量控制提供依据。方法采用RP-HPLC法,色谱柱为Phenome-nex C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水线性梯度洗脱,检测波长265 nm,流速0.8 mL·min-1,柱温35℃,并进行数字化评价。结果建立了栀子HPLC指纹图谱分析的研究方法,在指纹图谱中共有峰9个,并指认了3个色谱峰,指纹图谱的相似度均在0.98以上。结论建立的栀子药材HPLC指纹图谱可对栀子药材中的化学成分信息进行全面反映,适用于栀子药材的规范化种植及质量控制。

关 键 词:栀子  高效液相色谱法  指纹图谱  质量控制

HPLC fingerprint of Gardeniae fructus
REN Shu-juan,SUN Hui,DIN Ye,LI Wen-li. HPLC fingerprint of Gardeniae fructus[J]. West China Journal of Pharmaceutical Sciences, 2012, 27(3): 309-311
Authors:REN Shu-juan  SUN Hui  DIN Ye  LI Wen-li
Affiliation:1.Hunan University of Chinese Medicine,Changsha,Hunan,410208 P.R.China;2.Hunan Provincial Institute for Drug Control,Changsha,Hunan,410001 P.R.China)
Abstract:OBJECTIVE To establish the HPLC fingerprint of Gardeniae fructus,for its quality control.METHODS RP-HPLC method was applied to establish the chromatographic fingerprint.Chromatographic condition:Phenomenex-column,gradient elution with acetonitrile-water,the detection wavelength was at 265 nm,flow rate was at 0.8 mL·min-1,and the column temperature was 35 ℃.RESULTS HPLC-fingerprint of Gardeniae fructus from different areas was established,which contained 9 common peaks,and 3 peaks in the fingerprint were identified,and the similarities were over 98%.CONCLUSION The HPLC chromatographic fingerprint of Gardeniae fructus can express the chemical information completely and can be used for the standardization implantation and quality control of Gardeniae fructus.
Keywords:Gardeniae fructus  HPLC  Fingerprint  Quality control
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