一测多评法测定苦参中5种生物碱的含量

目的:在建立苦参中5种主要生物碱成分同时测定方法的基础上,建立5种生物碱一测多评测定方法,验证该方法在苦参含量测定中应用的可行性和技术适应性.方法:以苦参中5个主要生物碱成分为指标,建立氧化苦参碱与槐果碱、苦参碱、氧化槐果碱、槐定碱的相对校正因子,并考察了相对校正因子的重现性；同时采用外标法测定该5种生物碱的含量.结果:相对校正因子的重现性较好；21批苦参药材和饮片中5个生物碱的含量用一测多评法进行测定,其计算值与外标法实测值间无明显差异.结论:以同步测定氧化苦参碱、槐果碱、苦参碱、氧化槐果碱、槐定碱的一测多评法控制苦参药材和饮片的质量是可行的、准确的.

Simultaneous quantitative analysis of five alkaloids in Sophora flavescens by multi-components assay by single marker

Objective: To establish a new method for quality evaluation and validate its feasibilities by simultaneous quantitative assay of five alkaloids in Sophora flavescens.Method: The new quality evaluation method, quantitative analysis of multi-components by single marker (QAMS), was established and validated with S.flavescens.Five main alkaloids, oxymatrine, sophocarpine, matrine, oxysophocarpine and sophoridine, were selected as analytes to evaluate the quality of rhizome of S.flavescens, and the relative correction factor has good repeatibility.Their contents in 21 batches of samples, collected from different areas, were determined by both external standard method and QAMS.The method was evaluated by comparison of the quantitative results between external standard method and QAMS.Result: No significant differences were found in the quantitative results of five alkaloids in 21 batches of S.flavescens determined by external standard method and QAMS.Conclusion: It is feasible and suitable to evaluate the quality of rhizome of S.flavescens by QAMS.