芹菜素大鼠在体肠吸收动力学的研究

采用大鼠在体小肠单向灌流实验模型,以高效液相色谱法测定灌流液中药物浓度,研究芹菜素(apigenin,AP)肠吸收动力学.结果表明AP在整个肠段都有不同程度的吸收,且在十二指肠的吸收速率最快,其次空肠,最后回肠和结肠.AP在十二指肠和空肠内的吸收速率常数随着药物浓度的增加先增加后减小(P＜0.05),吸收机制可能有主动转运和异化扩散因素参与.AP在回肠和结肠内的吸收速率常数随着药物浓度的增加基本保持不变,其在回肠和结肠内的吸收机制为被动扩散.

Study on intestinal absorption kinetics of apigenin in rats

To establish an in situ single-way intestinal perfusion model, in order to study the intestinal absorption kinetics of AP.The concentration of AP in the perfusate was determined by HLPC.The results showed different AP concentrations in all intestinal segments, with the fastest absorption rate in duodenum, which was followed by jejunum, ileum and colon.In general, the constant absorption rate (K_a) of AP in duodenum and jejunum first increased and then decreased with the rise in drug concentration (P<0.05); the absorption mechanism may be related to active transport and facilitated diffusion factors.The constant absorption rate (K_a) of AP in ileum and colon generally kept unchanged with the rise diffusion in drug concentration, the absorption mechanism may be related to passive.