HPLC法测定蝙蝠葛碱与大鼠和人血浆蛋白的结合率

目的:建立测定蝙蝠葛碱的高效液相色谱(HPLC)法,并研究蝙蝠葛碱与大鼠和人血浆蛋白的结合率。方法:采用平衡透析法,HPLC法测定蝙蝠葛碱的浓度,计算蝙蝠葛碱大鼠及人血浆蛋白结合率。结果:透析外液中的蝙蝠葛碱检测浓度在0.24～15.10μg.mL-1范围内与其峰面积积分值呈良好线性关系;大鼠血浆中的蝙蝠葛碱检测浓度在0.24～15.10μg.mL-1范围内与其峰面积积分值呈良好线性关系;人血浆中的蝙蝠葛碱检测浓度在0.24～15.10μg.mL-1范围内与其峰面积积分值呈良好线性关系。蝙蝠葛碱在高、中、低浓度下,其大鼠血浆蛋白结合率分别为(69.63±1.30)%、(68.64±1.10)%、(72.54±0.50)%,人血浆蛋白结合率分别为(82.1±1.00)%、(84.96±0.70)%、(78.79±0.60)%。结论:HPLC法用于蝙蝠葛碱生物样品测定具有简便、灵敏与选择性高的特点;蝙蝠葛碱具有较强的血浆蛋白结合率,且大鼠和人血浆蛋白结合率具有种属差异性,人血浆蛋白结合率高于大鼠血浆蛋白结合率(P<0.05)。

Determination of Rat and Human Plasma Protein Binding Rate of Dauricine by HPLC

OBJECTIVE：To establish the HPLC method for the determination of dauricine,and to study the plasma protein binding rate of it.METHODS：The equilibrium dialysis and HPLC were used to determine the concentration of dauricine,and rat and human plasma protein binding rate of dauricine was calculated.RESULTS：The linear range of dauricine in extracellular fluid microdialysis was 0.24～15.10 μg·mL-1;in rat plasma was 0.24～15.10 μg·mL-1;in human plasma was 0.24～15.10 μg·mL-1;At high,middle and low concentrations,the rat plasma protein binding rates of dauricine were（69.63±1.30）%,（68.64±1.10）%,（72.54±0.50）%,respectively.The human plasma protein binding rates of dauricine were（82.1±1.00）%,（84.96±0.70）%,（78.79±0.60）%,respectively.CONCLUSIONS：HPLC method is simple,sensitive and selective for the determination of biological sample of dauricine.The plasma protein binding rate of dauricine is of a little high,and the human protein binding rate is higher than that of rat,there is difference among species（P0.05）.