| RP-HPLC法测定穿心莲注射液中苯甲醇的含量 |
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| 引用本文： | 叶静,毕开顺,宋肖炜,刘志红,陈晓辉. RP-HPLC法测定穿心莲注射液中苯甲醇的含量[J]. 中国药房, 2011, 0(31): 2929-2930 |
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| 作者姓名： | 叶静 毕开顺 宋肖炜 刘志红 陈晓辉 |
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| 作者单位： | 沈阳药科大学药学院; |
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| 基金项目： | 辽宁省教育厅高等学校科研项目(LT2010101) |
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| 摘 要： | 目的:建立测定穿心莲注射液中苯甲醇含量的方法。方法:采用反相高效液相色谱法。色谱柱为Kromasil C18(250mm×4.6mm,5μm),流动相为乙腈-水(35∶65),流速为0.5mL·min-1,检测波长为210nm,柱温为40℃,进样量为20μL。结果:苯甲醇检测浓度在0.595～11.900μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均加样回收率为99.0%,RSD=0.7%(n=9)。结论:本方法简便、准确、重复性好,可用于穿心莲注射液的质量控制。
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| 关 键 词： | 穿心莲注射液 苯甲醇 反相高效液相色谱法 含量测定 |
Content Determination of Benzyl Alcohol in Chuanxinlian Injection by RP-HPLC |
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| YE Jing,BI Kai-shun,SONG Xiao-wei,LIU Zhi-hong,CHEN Xiao-hui. Content Determination of Benzyl Alcohol in Chuanxinlian Injection by RP-HPLC[J]. China Pharmacy, 2011, 0(31): 2929-2930 |
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| Authors: | YE Jing BI Kai-shun SONG Xiao-wei LIU Zhi-hong CHEN Xiao-hui |
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| Affiliation: | YE Jing,BI Kai-shun,SONG Xiao-wei,LIU Zhi-hong,CHEN Xiao-hui(School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China) |
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| Abstract: | OBJECTIVE：To establish a method for the content determination of benzyl alcohol in Chuanxinlian injection.METHODS：RP-HPLC method was adopted.A Kromasil C18（250 mm×4.6 mm,5 μm） column was used with acetonitrile-water（35：65）as mobile phase at the flow rate of 0.5 mL·min-1.The detection wavelength was set at 210 nm and injection volume was 20 μL.RESULTS：The linear range of benzyl alcohol was 0.595～11.900 μg·mL-1（r=0.999 9）and the average recovery was 99.0%（RSD=0.7%,n=9）.CONCLUSION：The method is simple,accurate and reproducible for the quality control of Chuanxinlian injection. |
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| Keywords: | Chuanxinlian injection Benzyl alcohol RP-HPLC Content determination |
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