GC法测定香茅中香叶醇的含量

目的:建立测定香茅中香叶醇含量的方法。方法:采用超声提取法提取香茅中香叶醇,通过气相色谱法测定香叶醇的含量。色谱柱为Agilent Technologies DB-WAX(30m×0.25mm×0.25μm),以聚乙二醇(PEG-20M)为固定相,柱温为130℃,进样口温度为230℃,检测器温度为250℃,氮气(载气)流量为1.0mL.min-1,氢气(燃气)流量为40mL.min-1,空气(助燃气)流量为400mL.min-1。结果:香叶醇进样量在0.0153～0.1071μg范围内与峰面积积分值呈良好线性关系(r=0.9973);平均加样回收率为97.8%,RSD=1.68%(n=6)。结论:本方法简便、快速、准确、重复性好,可用于香茅的质量控制。

Content Determination of Geraniol in Cymbopogon citratus by GC

OBJECTIVE：To establish the method for the content determination of geraniol in Cymbopogon citratus.METHODS：Geraniol was extracted from C.citratus by ultrasonic extraction.The content of geraniol was determined by GC.Chromatographic column was Agilent Technologies DB-WAX（30 m×0.25 mm×0.25 μm） with PEG-20M as solid phase.The column temperature was 130 ℃,injector temperature was 230 ℃ and temperature of detector was 250 ℃.The flow rated of N2（carrier gas） was 1.0 mL·min-1 kPa.The flow rated of H2（fuel gas） was 40 mL·min-1 and that of air（assistant fuel gas） was 400 mL·min-1.RESULTS：The linear range of geraniol was 0.015 3～0.107 1 μg（r=0.997 3） with an average recovery of 97.8%（RSD=1.68%,n=6）.CONCLUSION：This method is simple,rapid and accurate,with good reproducibility.It can be used for quality control of C.citratus.