乳腺癌中PTEN蛋白表达及其与p27kip1和cyclin D1表达的相关性

目的 探讨抑癌基因PTEN蛋白在人乳腺癌中的表达情况,及其与抑癌基因p27^kiP1、癌基因cyclin D1表达的相关性。方法 应用免疫组织化学染色S-P法,检测6l例乳腺癌组织石蜡切片中PTEN蛋白、p27^kiP1和cyclin D1蛋白的表达情况,比较PTEN蛋白表达与临床病理指标的关系,及其与p27^kiP1、cyclin D1蛋白表达的相关性。结果 PTEN蛋白表达定位于细胞质。PTEN蛋白阴性占6．6％(4／61),表达减低者占41．0％(25／61),阳性表达者占52．5％(32／61)。PTEN与淋巴结状态、ER状态及局部复发、远处转移相关。单因素生存分析显示,PTEN蛋白高表达组的5年生存率(87．5％)高于低表达组(58．6％,P=0．0101)。PTEN蛋白表达与p27^kiP1、cyclin D1表达未显示有明显相关性。结论 乳腺癌患者PTEN蛋白低表达与预后不良有关,PTEN对乳腺癌肓一定的预后价值。在人乳腺癌中,p27^kiP1和cvclin D1可能不是PTEN的主要下游基因。

Expression of PTEN protein and its correlation with p27kip1 and cyclin D1 expression in primary breast cancer

Objective To study the expression of phosphatase and tensin homology deleted on chromosometen ten (PTEN) protein, a tumor suppressor gene in breast cancer and its correlation with p27 kip1 and cyclin D1 expression. Methods PTEN protein expression, p27 kip1 and cyclin D1 protein expression were detected by immunohistochemical method in paraffin sections from 61 women with primary breast cancer. PTEN protein expression was compared with clinico pathologic parameters as related to p27 kip1 and cyclin D1. Results PTEN, being shown in the cytoplasm, was negative in 6.6% (4/61), reduced in 41.0% (25/61) and positive in 52.5% (32/61) samples. PTEN expression level was correlated with axillary lymph node status, loss of estrogen receptor stain, recurrence and metastasis. On univariate analysis, the disease free survival rate of patients with higher PTEN expression(>50% cells stained) was better than those with lower expression( P =0.010 1). However, there was no correlation between p27 kip1 , cyclin D1 expression or PTEN expression. Conclusion PTEN, its lower expression being correlated with poor outcome of breast cancer patients, plays a prominent role in breast cancer. p27 kip1 or cyclin D1 may not be the primary downstream genes of PTEN in breast cancer. [