| TRAIL诱导胃癌细胞凋亡的实验研究 |
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| 引用本文： | 张玲,王庆才.TRAIL诱导胃癌细胞凋亡的实验研究[J].泰山医学院学报,2011,32(8):561-564. |
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| 作者姓名： | 张玲 王庆才 |
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| 作者单位： | 1. 泰山医学院附属泰山医院肿瘤内科,山东泰安,271000
2. 泰山医学院附属泰山医院消化内科,山东泰安,271000 |
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| 摘 要： | 目的研究TRAIL对胃癌细胞株MKN28细胞体外诱导凋亡的作用及机制,并研究此过程中Caspase-3的表达规律及与凋亡效应的相关性。方法培养细胞,MTT法分析细胞增殖抑制率及细胞存活率,流式细胞仪分析细胞凋亡及细胞周期,细胞免疫组化检测细胞Caspase-3的表达水平,AO/EB染色观察凋亡细胞。结果 TRAIL可明显抑制MKN28细胞的增殖,并可诱导细胞的凋亡。细胞阻滞于G0/G1期,Caspase-3在MKN28细胞中低表达,经TRAIL处理细胞后高表达。AO/EB染色后可见典型细胞凋亡特征。结论 TRAIL可诱导MKN28细胞株的凋亡,并呈剂量依赖性。TRAIL通过上调Caspase-3的表达,阻滞细胞周期,诱导MKN28细胞的凋亡。
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| 关 键 词： | TRAIL 凋亡 MKN28细胞 Caspase-3 |
Experimental studies of TRAIL protein on apoptosis of tumor cell |
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| ZHANG Ling,WANG Qing-cai.Experimental studies of TRAIL protein on apoptosis of tumor cell[J].Journal of Taishan Medical College,2011,32(8):561-564. |
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| Authors: | ZHANG Ling WANG Qing-cai |
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| Institution: | ZHANG Ling WANG Qing-cai(Central Hospital of Taian,Taian 271000,China) |
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| Abstract: | Objective:To investigate apoptosis induction of TRAIL and its mechanism in gastric cancer cell MKN28 line in vitro,and to study the express of Caspase-3 and its correlation with apoptosis.Methods: Their influence on cell proliferative activity was detected by MTT assay.The cell apoptosis and cell cycle was determined by flow cytometric(FCM) analysis.The expression of Caspase-3 was detected by immunohistochemistry.apoptosis was detected by AO/EB staining.Results: TRAIL could obviously inhibit the proliferati... |
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| Keywords: | TRAIL apoptosis cell MKN28 Caspase-3 |
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