掌叶大黄毛状根的诱导及其蒽醌类化合物产生的研究

目的:研究掌叶大黄毛状根的诱导及蒽醌类化合物的生产。方法:用发根农杆菌Agrobacterium rhizo-genesLBA9402和R1601感染掌叶大黄外植体。结果:2种发根农杆菌均能诱导掌叶大黄产生毛状根,LBA9402比R1601表现出较强的对掌叶大黄的感染能力。毛状根单克隆DH7 a(由R1601诱导)生长速度高于DH5 a,DH5 c(由LBA9402诱导),且明显比非转化根(NOR)快。DH5 a中蒽醌类化合物以大黄素为主,DH5 c则以大黄素甲醚含量最高,DH7 a中4种蒽醌———大黄酸、大黄素、大黄素甲醚和大黄酚含量相近,非转化根中大黄酚含量最高,而芦荟大黄素的含量在4种根中均较低。结论:本实验所建立的掌叶大黄毛状根培养系统,为研究大黄毛状根大量培养生产蒽醌类化合物奠定了基础。

Induction of hairy roots and anthraquinone production in Rheum palmatum

Objective: To study the induction of hairy roots and anthraquinone production in Rheum palmatum.Method: Leaf blades,hypocotyls and petioles of R.palmatum were infected by Agrobacterium rhizogenes LBA9402 and A.rhizogenes R1601,respectively.Result: Hairy roots were induced by two strains of A.rhizogenes, and LBA9402 showed stronger infective ability than R1601.Three clones of hairy roots were cultured.DH7a was induced by R1601,DH5a and DH5c were induced by LBA9402.DH7a grew faster than DH5a and DH5c,and all of them grew faster obviously than normal root(NOR).There were significant differences in anthraquinone composition and content among four kinds of roots.Emodin physcion and chrysophanol were predominant anthraquinone in DH5a,DH5c and NOR respectivly.Aloe-emodin content was the lowest in all root cultures compared with other four anthraquinones.Conclusion: The in vitro culture system of the established hairy roots laid a foundation for mass production of anthraquinone by hairy root culture.