| 成年大鼠脑室下区神经干细胞上N-甲基-D-天冬氨酸受体亚单位1的超微分布 |
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| 引用本文： | 李志宁,吕文龙,董红燕,董瑞国,徐铁军,Hongbin Fan. 成年大鼠脑室下区神经干细胞上N-甲基-D-天冬氨酸受体亚单位1的超微分布[J]. 中国神经再生研究, 2011, 6(28) |
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| 作者姓名： | 李志宁 吕文龙 董红燕 董瑞国 徐铁军 Hongbin Fan |
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| 作者单位： | 徐州医学院,连云港中医药高等职业技术学校，连云港222069；,徐州医学院人体解剖学与神经生物学教研室，徐州 221002,徐州医学院附属医院神经内科,徐州医学院人体解剖学与神经生物学教研室，徐州 221002,徐州医学院附属医院神经内科 |
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| 基金项目： | 江苏省高校自然科学基金（07KJB310119）；江苏省自然科学基金项目（BK2009087） |
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| 摘 要： | 以免疫电镜和免疫组化及免疫金-银双重标记技术，从超微结构方面对N-甲基-D-天冬氨酸受体亚单位1在神经干细胞上进行准确定位，结果证实脑室下区存在神经干细胞，是成年哺乳动物中枢神经系统重要的发生区，而N-甲基-D-天冬氨酸受体亚单位1的表达比Nestin高，且分布于神经干细胞胞膜、胞浆、以及粗面内质网、高尔基复合体上。
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| 关 键 词： | N-甲基-D-天冬氨酸受体亚单位1；脑室下区；神经干细胞；包埋前免疫电镜双标技术；超微结构 |
Subcellular distribution of N-methyl-D-aspartic acid receptor subunit 1 in neural stem cells within subventricular zone of adult rats |
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| Affiliation: | Zhining Li1,Wenlong Lü2,Hongyan Dong3,Hongbin Fan1,Ruiguo Dong1,Tiejun Xu3 1Department of Neurology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002,Jiangsu Province,China 2Lianyungang Higher Vocational Technical College of Traditional Chinese Medicine,Lianyungang 222069,China 3Department of Human Anatomy and Neurobiology,Xuzhou Medical College,China |
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| Abstract: | The subcellular localization of N-methyl-D-aspartic acid receptor subunit 1 in neural stem cells of the subventricular zone of adult rats was detected using electron microscopy, following immunohistochemistry and immunogold-silver double staining. Results confirmed the presence of neural stem cells in the subventricular zone, which is a key neurogenic region in the central nervous system of adult mammals. The expression of N-methyl-D-aspartic acid receptor subunit 1 was higher than that of nestin and mainly distributed in the cell membrane, cytoplasm, rough endoplasmic reticulum and Golgi complex of neural stem cells. |
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| Keywords: | N-methyl-D-aspartic acid receptor subunit 1 subventricular zone neural stem cells pre-embedding double labeled immunoelectron microscopy ultrastructure neural regeneration |
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