休克肠淋巴液对大鼠红细胞膜泵活性及氧自由基的影响

目的 观察休克肠淋巴液引流对失血性休克大鼠、休克肠淋巴液回输对正常大鼠红细胞膜泵活性及氧自由基的影响.方法 雄性Wistar大鼠24只,按随机数字表法分为假休克组、休克组、休克肠淋巴液引流组、休克肠淋巴液回输组,每组6只.休克组与引流组于麻醉和手术后复制失血性休克模型,引流组于休克1 h引流肠淋巴液;假休克组仅麻醉与手术;回输组于麻醉和手术后将引流的休克肠淋巴液回输.各组于休克3 h或相应时间点取腹主动脉血制备红细胞膜悬液,检测红细胞膜泵活性与自由基指标.结果 与假休克组比较,休克组红细胞膜Na+-K+-ATP酶(μmol·mg-1·h-1)、Ca2+-ATP酶(μmol·mg-1·h-1)、超氧化物歧化酶(SOD)活性(NU/mg)均显著降低(Na+-K+-ATP酶:0.039±0.011比0.068±0.010;Ca2+-ATP酶:0.035±0.016比0.087±0.015; SOD活性:0.785±0.289比1.202±0.328,P＜0.05或P＜0.01),丙二醛(MDA)含量(nmol/mg)显著升高(1.914±0.225比0.913±0.138,P＜0.01);与休克组比较,引流组红细胞膜Na+-K+-ATP酶活性(0.056±0.009)、Ca2+-ATP酶活性(0.079±0.025)、SOD活性(1.220+0.380)均显著升高,MDA含量(1.214±0.127)显著下降(P＜0.05或P＜0.01).与假休克组比较,回输组红细胞膜Na+-K+-ATP酶活性(0.050+0.013)、Ca2+-ATP酶活性(0.056±0.023)显著降低(均P＜0.05),SOD活性(0.862±0.288)有所降低(P＞0.05),MDA含量(1.456±0.270)显著升高(P＜0.01).结论 休克肠淋巴液回流是引起红细胞膜泵活性降低、加重红细胞膜自由基损伤的关键因素.

Effect of mesenteric lymph obtained during shock on pump activity and oxygen free radical on erythrocyte membrane of rat

Objective To observe the effects of mesenteric lymph drainage and transjusion on pump activity and oxygen free radical of erythrocyte membrane of rats following hemorrhagic shock or normal,respectively. Methods Twenty-four male Wistar rats were randomly divided into sham-shock group, shock group, shock mesenteric lymph drainage group (drainage group) and shock mesenteric lymph transfusion group (transfusion group) by randomization, with 6 rats in each group. Model of hemorrhagic shock was reproduced both in shock group and drainage group after anesthesia and operation, mesenteric lymph was drained 1 hour after shock in drainage group. Sham-shock group only received anesthesia and laparotomy,and the obtained mesenteric lymph was transfused in transfusion group. Blood was obtained from abdominal aorta, and red cell membrane suspension was prepared after 3 hours of shock or corresponding time, then the indices of erythrocyte membrane pump activity and oxygen free radical were determined. Results Compared with sham-shock group, the activity of Na+-K+-ATPase (μmol · mg-1 · h-1), Ca2+ATPase (μmol· mg-1· h-1) and superoxide dismutase (SOD) (NU/mg) of red cell membrane were decreased markedly (Na+-K+-ATPase: 0. 039+0. 011 vs. 0. 068+0. 010; Ca2+-ATPase: 0. 035±0. 016 vs. 0. 087±0. 015; SOD: 0. 785±0.289 vs. 1. 202±0. 328, P＜0. 05 or P＜0. 01), and the content of malondialdehyde (MDA, nmol/mg) was increased markedly in shock group (1. 914±0. 225 vs. 0. 913±0. 138, P＜0. 01).Compared with shock group, the activity of Na+-K+-ATPase (0. 056 ± 0. 009), Ca2+-ATPase (0. 079 ±0. 025) and SOD (1. 220±0. 380) of red cell membrane were increased in drainage group, and the level of MDA (1. 214±0. 127) was decreased markedly (P＜0. 05 or P＜0. 01). Compared with sham-shock group,the activity of Na+-K+-ATPase (0. 050 ± 0. 013), Ca2+-ATPase (0. 056 ± 0. 023) of red cell membrane in transfusion group were significantly lower (both P＜0.05), the content of MDA (1. 456± 0. 270) was significantly higher (P＜0. 01), respectively, the activity of SOD (0. 862± 0. 288) showed a lowering trend,but there was no statistical significance (P＞0. 05). Conclusion Mesenteric lymph return is a key factor in decreasing pump activity while aggravating free radical damage injury of erythrocyte membrane.