Preparation and some properties of highly purified human serum kallikrein. |
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Authors: | G A Yarovaya V L Dotsenko V N Orekhovich J Kawiak M Kawalec |
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Affiliation: | 1. Chair of Biochemistry of the Central Institute for Advanced Training of Physicians, MoscowU.S.S.R.;2. Medical Center of Post-graduate Education, Cytophysiological Laboratory, WarsawPoland |
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Abstract: | A 3000--6000-fold purified kallikrein was obtained from human serum in 10--25% yield by chromatography on QAE-Sephadex A-50, Molselect CM-50 and on soybean trypsin inhibitor (SBTI)-AH-Sepharose 4-B. The enzyme had a specific activity of 14--23 U, as measured by BAEE hydrolysis. Some properties of highly purified kallikrein are described. |
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