两种基因芯片杂交法荧光信号的比较研究

目的:探讨样品在流动态和静置态两种杂交环境下分别与基因芯片进行杂交,比较两种方法对杂交信号的影响。方法:人红白血病K562细胞mRNA经限制性荧光标记后在循环流动的杂交环境和传统静置的杂交环境中分别与K562表达谱芯片杂交,在同等条件下进行杂交后的清洗和芯片扫描检测。结果:流动态的样品相对于静置态的样品与芯片杂交产生的杂交信号荧光背景低,灵敏度和信噪比高,并且杂交点的同一性好。结论:样品在流动态与芯片杂交得到的杂交效果要好过传统的杂交方法,从而为基因芯片的实际应用提供了新的研究方法和思路。

Comparative study on effect of two hybridization methods for enhancing the signal of cDNA microarray

Objective To compare the effect of stationary and rotating microchamber in enhancing the fluorescence signal of gene expression profile after microarray hybridization. Methods cDNA probes were made from mRNA of K562 cells by restriction fluorescent labeling method. The labeled samples were examined by microarray in the stationary and rotating microchamber, followed by washing and scanning under the same conditions. Result The rotating, circular hybridization method showed superior results with lowered background and enhanced signal-to-noise ratio and sensitivity. Conclusions Transporting the samples in a set of rotating, circular mode can yield significantly larger hybridization intensities. The rotating hybridization method can speed up the further application of microarray technology.