抗口腔支原体、精氨酸支原体单克隆抗体的制备及其应用方法的初步研究

用口腔支原体、精氨酸支原体抗原免疫ＢＡＬＧ／ｃ小鼠，取其脾细胞，与小鼠骨髓瘤细胞（Ｓｐ２／０）融合，获得了７株稳定分泌抗口腔支原体单克隆抗体（ＭｃＡｂ），２株分泌抗精氨酸支原体ＭｃＡｂ的杂交瘤细胞株。间接ＥＬＩＳＡ测定其抗体效价可达１：１０３～１：１０７；９株ＭｃＡｂ中１株为ＩｇＧ２ａ，其余均为ＩｇＧ１。初步建立了ＥＬＩＳＡ检测支原体的方法。

Preparation and Preliminary Application of Monoclonal Antibodies against M.orale and M.arginine

Monoclonal antibodies(McAbs) were prepared by fusing of Sp2/0 cells with spleen cells of BALB/c mice immunized with the antigens of M.orale and M.arginine respectively.Antibodies in hybrid culture supernatants were detected by ELISA,and positive hybrids were cloned by the technique of limiting dilutions.Nine strains of hybridoma cell lines have been obtained,of which seven strains secreted anti-M. orale antibodies and two strains secreted anti-M.arginine antibodies.One of the immunoglobulin class/subolass of McAbs was IgG2a and the others belinged to IgG2.ELISA was used for identifying titers of nine McAbs which were from 1:103 to 1:107.The methods,which were used to detect M.orale by sandwich ELISA and to detect M.argininge by competitive ELISA,have been established.