氧化低密度脂蛋白对血管平滑肌细胞早期凋亡的影响

目的：观察低密度脂蛋白(ox—LDL)对培养血管平滑肌细胞(VSMC)凋亡的影响，探讨ox—LDL致VSMC调亡的相关机制。方法：体外培养Wistar大鼠VSMC并确认细胞指数生长期，用苔盼蓝拒染计数方法观察ox—LDL刺激VSMC对增殖的影响，流式细胞分析检测ox—LDL刺激VSMC凋亡以及COx—2抑制剂NS—398对凋亡的影响。结果：体外培养的VSMC正常生长后，用35mg儿和50mg／Lox—LDL可使培养VSMC的生长期提前到12—24h，表现出明显细胞增殖；NS—398干预ox—LDL组VSMC增殖受到减弱。流式细胞分析ox—LDL组VSMC早期凋亡率较对照组明显增加，而NS—398、ox—LDL刺激VSMC凋亡阳性率亦较对照组明显增加。结论：ox—LDL刺激可使培养VSMC表现明显细胞增殖，虽NS—398可减弱VSMC的增殖，但却协同ox—LDL导致凋亡作用的增强。

Effect of oxidated low-density lipoprotein on early apoptosis of vascular smooth muscle cell

Objective:To investigate the apoptosis in vascular smooth muscle cell (VSMC) induced by oxidated lowdensity lipoprotein cholesterol (oxLDL),and to discuss the relative mechanism of the apoptosis of VSMC.Methods:The growth index of VSMC of Wistar rat was determined.By using trypan blue to count unstaining cells,the proliferative effect of oxLDL on culturing VSMC was observed.With flow cytometer,the apoptosis of VSMC induced by oxLDL and the inhibitory effect of cyclooxygenase2 (COX2) inhibitor NS398 on apoptosis were determined.Results:After VSMC grew normally in culture, oxLDL in concentrations of 35 mg/L and 50 mg/L could advance the growth period of VSMC to between 12 and 24 hours,and the proliferation of VSMC were obvious.The proliferation of VSMC induced by oxLDL were inhibited by NS398.The rate of apoptosis of VSMC in oxLDL and NS398 groups were higher than that of normal control.Conclusion:oxLDL could induce an obvious proliferation of VSMC in culture. Although NS398 can decrease the proliferation of VSMC,it could enhance oxLDL to induce apoptosis.