NK／T细胞对肺腺癌患者生存与预后的影响

目的研究NK／T细胞在肺腺癌组织中的浸润程度及对患者生存与预后的影响。方法将CD8作为细胞毒T淋巴细胞（CTL）的标记物，CD56作为自然杀伤细胞（NK）的特异性标记物，应用免疫组织化学方法检测肿瘤组织中NK／T细胞的分布和浸润情况。结果48例肺癌中，CTL无-轻度浸润的29例，5年生存率为20％；显著浸润的19例，5年生存率为57％；NK细胞无-轻度浸润的34例，5年生存率为23％，显著浸润的14例，5年生存率为64％：NK／T均显无-轻度浸润的36例，5年生存率为25％，显著浸润的12例，5年生存率为66％。经Log-rank检验，NK／T显著浸润组和无-轻度浸润组二者的5年生存率之间存在显著性差异（P〈0．05），有统计学意义。结论肺腺癌组织中NK／T细胞显著浸润组的预后和生存时间明显好于轻度浸润组。其机理与机体细胞免疫有关。

Impact of NK/T cell on the survival and prognosis of primary adenocarcinoma patients

Objective To investigate the degree of infiltration of NK and T cell in primary pulmonary adenocarcinoma and the impact on the survival and prognosis of the patients. Methods CD8 was used as the markers of cytoxic T lymphocyte （CTL） and CD56 as the markers of natural killer（NK） for detection of distribution and infiltration of NK/T cells in tumor tissue after stained immunohistochemically. Results Out of the 48 pulmonary adenocarcinoma patients there no infiltration in tissue by CTL was observed in 29 patients with a five-year survival rate of 20% ; while in 19 with marked CTL infiltration, the five - year survival rate was 57% ;of the 34 patients without infiltration by NK cells with a five - year survival rate of 23 % and the five -year survival rate was 64% in another 14 ptients with marked infiltration in the tissue by NK cells; The five- year survivl rate of 36 patients without infiltration in the tissue either by NK and T cells was 25%, and tthat in another 12 patients with marked infiltration was 66%. Significant statistical diffeences were observed between the NK/T infiltration group and without infiltration group （P 〈 0.05 ）. Conclusion The survival and prognosis of adenocarcinoma patients with marked infiltrtion in tissues is appraently better than that of without or light infiltration group. The degree of NK and T infiltration is positively correlated with the prognosis and survival time of pulmonary adenocarcinoma patients, the mechanism might be associated with cellular immunity.