缺氧对牛视网膜血管内皮细胞整合素受体αvβ3表达及功能的影响

目的 探讨缺氧对牛视网膜血管内皮细胞整合素受体αvβ3表达及功能的影响。 方法 以玻璃粘连蛋白(Vn)包被培养皿，将牛视网膜血管内皮细胞置入正常及缺氧条件下，孵育12、24、48、60、72 h后以酶联免疫吸附方法检测细胞表面整合素受体αvβ3的表达，并通过细胞粘附分析检测其功能。 结果 缺氧条件下，αvβ3的表达逐渐增加，在48h达到顶峰，在60、72h仍较正常环境高。缺氧24h后，牛视网膜血管内皮细胞对细胞外基质Vn的粘附明显增加。 结论 缺氧可上调培养的牛视网膜血管内皮细胞αvβ3的表达，从而促进内皮细胞的粘附。 （中华眼底病杂志，2004，20：361-363）

Effect of hypoxia on expression and function of integrin receptor αvβ3 of bovine retinal vascular endotheliocytes

Objective To investigate the effect of hypoxia on the exp ression and function of integrin receptor αvβ3 of bovine retinal vascular endotheliocytes. Methods Bovine retinal vascular endotheliocy tes in the culture dishes coated by vitronectin was put into the normal and hypoxemic condition, respectively. Enzyme linked immunosorbent assay and cell adhesion analysis were used to detect the expression and function of integrin receptor αvβ3 in bovine retinal vascular endotheliocytes, respectively. Results Under the condition of hypoxia, the expression of αvβ3 increased gradually, and reached the peak at the 48th hour. The expression of αvβ3 at the 60th and 72nd hour in hypoxia group was higher than that in the normal group. Bovine retinal vascular endotheliocytes absorbed more Vn of extra-cellular matrixes (ECM) after cultured under hypoxemic condition for 24 hours.Conclusion Hypoxia may up-regulate the expression of αvβ3, which promote the adsorbability of endotheliocytes.(Chin J Ocul Fundus Dis，2004，20：360-363)