D2S140基因多态性与2型糖尿病的相关性研究

目的：探讨D2S140基因多态性与2型糖尿病的关系。方法：运用聚合酶链反应（PCR）技术，结合短串联重复序列（STR）多态性标记的方法，检测了103例2型糖尿病患者和105例正常对照者的D2S140等位基因及基因型，结果：发现7种等 位基因。等位基因2与2型糖尿病呈显著性正相关（RR＝13。76，P＜0．001），等位基因6与2型糖尿病呈显著性负相关（RR＝0．25，P＜0．001）。多因素非条件Logistic回归分析表明，D2S140等位基因2是2型糖尿病的独立危险因素，其相对危险度明显高于环境因素。结论：DS2S140基因多态性与中国人2型糖尿病相关。

Relationship between D2S140 gene polymorphism and type 2 diabetes mellitus

Objective To determine the relationship between the polymorphism of D2S140 gene and type 2 diabetes mellitus.Methods D2S140 region polymorphism were analyzed with the polymerase chain reaction and short tandem repeat methods in 103 subjects with type 2 diabetes mellitus and 105 nondiabetic subjects.All subjects were biologically unrelated Chinese without a family history of DM.Results There were 7 alleles ranging from 151bp to 163bp in all subjects.The frequencies were 0.01 to 0.37,2 alleles were more common in diabetic patients than in nondiabetic subjects (RR=16.04, P <0.001) and 6 alleles were less frequent in diabetic patients than in nondiabetic subjects (RR=0.23, P <0.001).Logistic regression analysis showed that the 2 alleles of D2S140 gene are main risk factors for type 2 diabetes mellitus.Conclusion Polymorphic microastellite repeat marker at the D2S140 gene locus is positively associated with type 2 diabetes mellitus in Chinese.