Production of a monoclonal antibody with specificity for deoxynivalenol, 3‐acetyldeoxynivalenol and 15‐acetyldeoxynivalenol |
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| Authors: | Maurice J Nicol Denis R Lauren Christopher O Miles William T Jones |
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| Institution: | 1. The Horticulture and Food Research Institute of New Zealand Ltd , Palmerston North, New Zealand;2. The Horticulture and Food Research Institute of New Zealand Ltd , Hamilton, New Zealand;3. New Zealand Pastoral Agriculture Research Institute Ltd , Hamilton, New Zealand |
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| Abstract: | Monoclonal antibodies reactive with deoxynivalenol were generated following the immunization of mice with a deoxynivalenol‐mouse serum albumin conjugate. One of the anti‐deoxynivalenol monoclonal antibodies, designated C6–1, exhibited cross‐reactivity with 3‐acetyldeoxynivalenol and 15‐acetyldeoxynivalenol but not with nivalenol, T‐2 tetraol or scirpentriol. An indirect competitive ELISA based on this monoclonal antibody gave 50% inhibition values of 0–6 μg ml‐1 for deoxynivalenol, 0–2 μg ml‐1 for 15‐acetyldeoxynivalenol and 10 μg ml‐1 for 3‐acetyldeoxynivalenol. |
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| Keywords: | Deoxynivalenol 3‐acetyldeoxynivalenol 15‐acetyldeoxynivalenol ELISA mouse serum albumin |
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