| 积雪草苷对烫伤创面成纤维细胞及结缔组织生长因子表达的影响 |
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| 引用本文： | 姜润松,赵雄,林隆,赵旭飞,顾伟忠,岳晓洁,贺敏. 积雪草苷对烫伤创面成纤维细胞及结缔组织生长因子表达的影响[J]. 浙江医学, 2013, 0(23): 2054-2056 |
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| 作者姓名： | 姜润松 赵雄 林隆 赵旭飞 顾伟忠 岳晓洁 贺敏 |
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| 作者单位： | [1]浙江大学医学院附属儿童医院整形外科,杭州310003 [2]杭州市红会医院,杭州310003 |
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| 基金项目： | 浙江省中医药科学研究基金（2011ZA066） |
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| 摘 要： | 目的探讨积雪草苷对烫伤创面成纤维细胞及结缔组织生长因子（CTGF）表达的影响。方法将30只SD大鼠按随机数字表法进行分组，选取6只大鼠不予烫伤，其余进行烫伤建模，采用积雪草苷和凡士林对烫伤创面组织进行同体对照研究，HE染色评估成纤维细胞变化，免疫组织化学法检测CTGF蛋白表达情况，并用透射电镜观察成纤维细胞微观结构改变。结果积雪草苷治疗组大鼠伤后第14天创面CTGF含量高于对照组（P〈0．05），伤后第21天创面CTGF含量低于对照组（P〈0.05）。成纤维细胞HE染色的大体形态及电镜观察呈现双相动态变化。结论积雪草苷可能对成纤维细胞增殖及CTGF表达存在双相调节作用，对促进创面少瘢痕愈合有一定作用。
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| 关 键 词： | 积雪草苷 成纤维细胞 结缔组织生长因子 |
Effects of Asiaticoside on the fibroblasts of burned rats and the expression of connective tissue growth factor |
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| Affiliation: | JIANG Runsong, ZHAO Xiong, LIN Long, et al.( Department of Reconstructive Plastic Surgery, the Children＇s Hospital of Zhejiang University School of Medicine, Hangzhou 310003, China) |
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| Abstract: | Objective To investigate the effects of Asiaticoside on the fibroblasts of burned rats and the expression of connective tissue growth factor. Methods 30 SD rats were randomized into treatment group（n=24） and control group（n=6） ac- cording to random number table. Asiaticoside and Vaseline were used to treat the burn wound respectively in two groups. Mor- phological changes of fibroblasts were detected with HE staining and transmission electron microscopy, while the expression of CTGF protein was detected by immunohistochemistry. Results The expression of CTGF was significant increased in treatment group 14 days after burn, while significant decreased 21 days after（P〈0.05）. Fibroblasts showed biphasic morphological change under light microscope and electron microscopy. Conclusion Asiaticoside had the biphasic regulate effect on the fibroblasts and expression of CTGF. It maybe promote wound healing with less scar. |
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| Keywords: | Asiaticoside Fibroblast Connective tissue growth factor |
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