PI3K/AKT信号通路与PTEN在非小细胞肺癌表达中的相关性

目的研究表皮生长因子受体(epidermal growth factor receptor,EGFR)、磷酸化AKT蛋白(phophorylatedAKT,p-AKT)及PTEN基因(phosphatase and tensin homology deleted on chromosome,PTEN)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及意义。方法 采用免疫组织化学Maxvision法检测EGFR、p-AKT及原位杂交法检测PTEN在66例NSCLC和10例正常肺组织中的表达,并分析两者的相关性及其与临床病理特征的关系。结果 EGFR、p-AKT和PTEN在NSCLC中的阳性率分别为69.7%、75.8%、33.3%,在NSCLC中,EGFR的高表达与分化程度、淋巴结转移及TNM分期密切相关(P<0.05);p-AKT的高表达与分化程度、淋巴结转移及TNM分期密切相关(P<0.05)。PTEN在NSCLC中的阳性表达率明显下调,其失表达与分化程度、淋巴结转移、TNM分期和病理类型密切相关(P<0.05)。EGFR与p-AKT表达呈正相关(P<0.05),EGFR与PTEN表达呈负相关(P<0.05),p-AKT与PTEN表达呈负相关(P<0.05)。结论 EGFR和p-AKT在NSCLC的高表达和PTEN基因失表达,可能促进了NSCLC的发生、发展及侵袭转移。可为NSCLC基因靶向治疗提供理论依据。

Correlation of PI3K/AKT signal transduction pathway and PTEN expression in non-small cell lung cancer

Objective To investigate the expression of epidermal growth factor receptor （EGFR） ,phophorylated AKT（P-AKT） and phosphatase and tensin homology deleted on chromosome （ PTEN ） in non-small cell lung cancer （ NSCLC ）. Methods The expressions of ECFR, P-AKT proteins and PTEN gene in 66 cases of NSCLC and 10 cases of normal lung tissue were detected by immunohistochemistry Maxvision method and in situ hybridization, respectively. The correlation of the expressions of EGFR, P-AKT and PTEN were analyzed. Results The positive expression rate of EGFR, P-AKT and PTEN in NSCLC was 69.7% , 75.8%, 33.3%, respectively. The over-expression of EGFR and P-AKT was significantly correlated with the differentiation extent of cancer tissue, metastasis of lymph nodes and TNM stages （ P 〈 0.05 ）. The loss of expression of PTEN had correlation with the differentiation extent of cancer tissue, metastasis of lymph nodes, TNM stages and pathologic type （ P 〈 0.05 ）. The expression of EGFR was positively correlated with P-AKT; while the expression of ECFR and P-AKT was negatively correlated with that of PTEN （ P 〈 0.05 ）. Conclusion Over- expression of ECFR,P-AKT and low expression of PTEN is closely correlated to the development, invasion and metastasis of NSCLC, indicating that these proteins might be used as potential therapeutic targets for NSCLC.