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HPLC法测定黄芪中槲皮素和山奈酚的含量
引用本文:典灵辉,吴铁,吕应年. HPLC法测定黄芪中槲皮素和山奈酚的含量[J]. 现代医药卫生, 2007, 23(17): 2550-2551
作者姓名:典灵辉  吴铁  吕应年
作者单位:广东医学院,广东,东莞,523770
摘    要:目的:建立黄芪中槲皮素和山奈酚含量的测定方法。方法:采用反相高效液相色谱法(HPLC)测定不同来源黄芪中槲皮素和山奈酚含量。结果:不同来源样品中2种黄酮苷元的定量分析结果分别为槲皮素平均回收率97.88%,RSD=1.07%;山奈酚平均回收率为99.83%,RSD=1.10%。结论:黄芪中含有槲皮素和山奈酚,其含量随产地而变化。

关 键 词:高效液相色谱法  黄芪  槲皮素  山奈酚
文章编号:1009-5519(2007)17-2550-02
修稿时间:2007-05-24

Determination of the contents of quercetin and kaempferol in astragalus membranaceus(fish.) bunge by HPLC
DIAN Ling-hui,WU Tie,LU Ying-nian. Determination of the contents of quercetin and kaempferol in astragalus membranaceus(fish.) bunge by HPLC[J]. JOURNAL OF MODERN MEDICINE & HEALTH, 2007, 23(17): 2550-2551
Authors:DIAN Ling-hui  WU Tie  LU Ying-nian
Affiliation:Guangdong Medical College, Guangdong 523770, China
Abstract:Objective:To establish a HPLC method for the determination of the contents of quercetin and kaempferol in astragalus membranaceus(fish.)bunge.Methods:The determination of two kinds of flavonoid aglycones was performed by HPLC.Results:The average recovery of quercetin was 97.88%,RSD=1.07%.The average recovery of kaempferol was 99.83%,RSD=1.10%.Conclusion:There are quercetin and kaempferol in astragalus membranaceus(fish.)bunge,and their contents change with various origins.
Keywords:HPLC  Astragalus membranaceus(fish.)bunge  Quercetin  Kaempferol
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