| 人组织因子途径抑制因子重组蛋白表达菌株的建立 |
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| 引用本文： | 冷希岗,王传华,宋丽萍,李晓征,马洁,王彭延.人组织因子途径抑制因子重组蛋白表达菌株的建立[J].生物医学工程学杂志,2003,20(2):314-317. |
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| 作者姓名： | 冷希岗 王传华 宋丽萍 李晓征 马洁 王彭延 |
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| 作者单位： | 1. 中国医学科学院、中国协和医科大学生物医学工程研究所,天津,300192;南开大学生物活性材料教育部重点实验室,天津,300071
2. 中国医学科学院、中国协和医科大学生物医学工程研究所,天津,300192 |
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| 基金项目： | 国家自然科学基金资助项目 (3 9670 2 2 2 )，天津市自然基金资助项目 (993 60 45 11) |
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| 摘 要： | 组织因子途径抑制因子是机体凝血过程的主要抑制因子，在血栓形成性疾病的防治中具有广阔的应用前景。本研究采用pGEX—2T为表达载体、大肠杆菌细胞为表达宿主进行了人组织因子途径抑制因子重组蛋白的制备。制备的重组蛋白产量较高，纯化过程简单，且具有良好的抑制组织因子的功能。
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| 关 键 词： | 组织因子途径抑制因子 血栓形成性疾病 凝血系统 表达载体 大肠杆菌细胞 跨膜糖蛋白 |
Generation of Recombinant Human Tissue Factor Pathway Inhibitor in E.coli |
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| Leng Xigang , Wang Chuanhua Song Liping Li Xiaozheng Ma Jie Wang Pengyan.Generation of Recombinant Human Tissue Factor Pathway Inhibitor in E.coli[J].Journal of Biomedical Engineering,2003,20(2):314-317. |
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| Authors: | Leng Xigang Wang Chuanhua Song Liping Li Xiaozheng Ma Jie Wang Pengyan |
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| Institution: | Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Bioactive Materials Key Lab of Ministry of Education, Nankai University, Tianjin. |
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| Abstract: | Tissue factor pathway inhibitor (TFPI) is one of the major physiological inhibitors of the human blood coagulation cascade and may have great potential in the prevention and therapy of diseases caused by thrombus formation. In this study, recombinant human tissue factor was generated in E.coli containing a recombinant vector constructed by inserting TFPI cDNA into pGEX 2T vector. The generated recombinant TFPI (rTFPI) could be simply purified with glutathione agarose affinity method and maintained its biological function in terms of inhibition of tissue factor and factor Xa. |
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| Keywords: | Tissue factor pathway inhibitor Recombinant protein E coli |
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