Fractionation of Theiler's virus-infected BHK21 cell homogenates: isolation of virus-induced membranes |
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| Authors: | G Frankel Y Lorch P Karlik A Friedmann |
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| Institution: | 1. Department of Pharmacy, Birla Institute of Technology & Science—Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet, Hyderabad 500078, Telangana State, India;2. Regional Agricultural Research Station, Professor Jayashankar Telangana State Agricultural University, Palem 509 215, Mahaboobnagar District, Telangana State, India;3. Department of Chemistry and Materials Science, Tokyo Institute of Technology, O-okayama, Meguro, Tokyo 152-8551, Japan;1. Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav St., 1000 Sofia, Bulgaria;2. Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 4, A-8010 Graz, Austria;3. Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Universitätsplatz 1, A-8010 Graz, Austria;4. Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University of Sofia, 2 Dunav St., 1000 Sofia, Bulgaria;1. Department of Pharmacy, Guiyang University of Chinese Medicine, Guiyang 550025, PR China;2. First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China;1. Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 S. Wood Street, Chicago, IL 60612, United States;2. Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, United States;3. Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, 833 S. Wood Street, Chicago, IL 60612, United States;1. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, People''s Republic of China;2. Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, 40225 Duesseldorf, Germany;1. Department of Pharmacognosy, Faculty of Pharmacy, Port Said University, Port Said 42526,Egypt;2. Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia;3. Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Assuit Branch, Assuit 71524, Egypt;4. Department of Pharmacognosy, Faculty of Pharmacy, Assuit University, Assuit 71526, Egypt;5. Garden for Medicinal Plants, Graduate School of Biomedical Sciences, Nagasaki University, Bunkyo-machi 1-14, Nagasaki 852-8521, Japan |
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| Abstract: | A purified fraction containing unique membranes entrapping virions was isolated from homogenates of cells infected with the DA strain of Theiler's virus, after high-speed centrifugation through a sucrose gradient. This fraction, sedimented at 45-50% sucrose, was only found in cells infected with the DA strain but not in cells infected with the GDVII strain of Theiler's virus or in mock-infected cells. Immunogold staining of the membranes entrapping virions, using antivirus IgG antibodies, revealed that the membranes entrapping virions did not incorporate viral capsid antigens. |
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