Neurotoxic effects of chronic restraint stress in the striatum of methamphetamine-exposed rats |
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Authors: | M S Quinton B K Yamamoto |
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Institution: | (1) Laboratory of Neurochemistry, Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Room L-613, 715 Albany Street, Boston, MA 02118, USA |
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Abstract: | Rationale Stress is a common experience in drug abusers. Methamphetamine (METH) is an abused psychostimulant that damages dopamine and
serotonin terminals through pro-oxidant mechanisms and glutamate-mediated excitotoxicity. Both METH and stress increase dopamine
and glutamate release in the striatum. Since dopamine inhibits striatal glutamate release and METH depletes dopamine, stress-induced
glutamate release may be disinhibited after METH exposure.
Objective We examined if repeated stress would worsen excitotoxic damage to the striatum after METH pretreatment.
Materials and methods In vivo microdialysis was used to examine stress-induced striatal glutamate release in rats pre-exposed to METH (7.5 mg/kg × 4
injections) or saline. The effects on striatal DA, serotonin, DAT, SERT, and spectrin proteolysis produced by chronic restraint
stress (CRS, 6 h/day for 21 days) in the presence or absence of corticosterone synthesis inhibition by metyrapone (50 mg/kg)
beginning 7 days after METH were also examined.
Results Stress-induced glutamate release was augmented in rats pre-exposed to METH. CRS 7 days after METH enhanced METH-induced DAT
depletions from 23 to 44% in the nonstressed versus stressed rats, respectively. Striatal SERT and serotonin tissue content
were decreased by 51 and 36%, respectively, in rats exposed to both METH and CRS but was unchanged by either treatment alone.
Spectrin proteolysis was increased by 53% in rats treated with both METH and CRS but was unaffected by either treatment alone.
Metyrapone blocked the effects of CRS on METH-induced depletions of SERT but not DAT.
Conclusions Exposure to chronic stress depleted striatal dopamine and serotonin terminal markers possibly through excitotoxic mechanisms
in METH-treated rats. |
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Keywords: | Methamphetamine Metyrapone Chronic restraint stress Glutamate Excitotoxicity Dopamine transporter Serotonin transporter Spectrin proteolysis |
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