ICR胎鼠原代神经元细胞培养及鉴定

目的 建立较理想的胎鼠神经元细胞体外原代培养方法。方法 取13.5 d ICR胎鼠的大脑皮层，经剪碎消化得到单细胞悬液进行培养，观察细胞形态并作PCR及Western blotting等进行神经元相关的基因及蛋白Tuj1和Map2的鉴定。结果 细胞生长状态良好，细胞胞体明显，周围有明亮光晕，细胞突触交错形成网络样，通过PCR及Western blotting验证证明所分离培养的细胞是神经元细胞。结论 本培养方法简单易行，可获得典型和纯度较高的ICR胎鼠大脑皮层神经元细胞。

Culture and identification of primary neurons isolated from embryonic ICR mice

Objective To establish a favorable primary culture technique for neurons isolated from embryonic ICR mouse cortical tissues. Methods The cortex of embryonic ICR mice aged 13.5 d was isolated, mechanically dissected and digested, and was proceeded to culture. The morphology of neurons was observed, and PCR and Western blotting were applied to identify the expression of Tuj1 and Map2 gene and protein in neurons. Results Cells grew well, with distinct cell body and surrounding bright halation, and there was typical nerve fiber network of synapses. The isolated and cultured cells were confirmed as neurons by PCR and Western blotting. Conclusion This technique is an easy and practical tool for the primary culture of embryonic ICR mouse cortical neurons with high purity.