曲格列酮对大鼠垂体腺瘤GH3细胞CD147、TGF-β1和MMP-9表达的影响

目的   探讨过氧化物酶体增殖激活物受体γ(PPAR-γ)的配体曲格列酮(TGZ)对大鼠垂体腺瘤GH3细胞系白细胞分化抗原147(CD147)、转化生长因子β1(TGF-β1)和基质金属蛋白酶9(MMP-9)表达的影响。方法   GH3细胞分为空白对照组和实验组(TGZ浓度分别为1×10-3、1×10-4和1×10-5 mol／L)。TGZ作用GH3细胞48h后，分别采用Western blot和荧光定量PCR检测各组细胞中CD147、TGF-β1、MMP-9蛋白和mRNA的表达。结果   各组细胞CD147、TGF-β1、MMP-9蛋白和mRNA的表达与空白对照组相比，均有统计学差异(P均<0.05)，而且TGZ浓度越高，CD147、TGF-β1、MMP-9蛋白和mRNA的表达量越低。结论   PPAR-γ配体TGZ能降低CD147、TGF-β1、MMP-9的表达，并可能通过该作用减弱GH3细胞的侵袭力。

Effects of troglitazone on the expressions of CD147, TGF-β1 and MMP-9 in GH3 cells

Objective   To explore the effect of troglitazone(TGZ), a ligand for peroxisome proliferator-activated receptor γ(PPAR-γ), on the expressions of cluster of differentiation 147(CD147), transforming growth factor beta 1(TGF-β1) and matrix metalloproteinase 9(MMP-9) in rat GH-secreting GH3 cells. Methods   GH3 cells were devided into control group and experimental groups (1×10-3, 1×10-4 and 1×10-5 mol／L TGZ). After treated with TG2 for 48 hours, expressions of CD147, TGF-β1 and MMP-9 at protein and mRNA levels were detected by Western blot and Real-time PCR respectively. Results   The expressions of CD147, TGF-β1 and MMP-9 at protein and mRNA levels in all the TGZ treated groups showed significant differecnces compared with control group(all P<0.05). Moreover, expressions of the above three indicators decreased both at protein and mRNA levels when TGZ concentration was increased. Conclusion   TGZ can inhibit expressions of CD147, TGF-β1 and MMP-9, which   might relieve the invasiveness of GH3 cells.