TLC及HPLC法监测白带丸中黄柏的质量

目的黄柏与关黄柏为2种不同的药材,但在使用过程中时有混淆。该文建立薄层色谱和高效液相色谱法监测白带丸中黄柏的质量。方法薄层色谱法采用硅胶G薄层板,以苯—异丙醇—乙酸乙酯—甲醇—浓氨试液(12∶3∶6∶3∶1)为展开剂展开。HPLC法采用Lichrospher C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.05 mol.L-1磷酸二氢钾溶液(氢氧化钠试液调节pH值至5.0)(25∶75)为流动相,检测波长为346 nm,流速1.0 mL.min-1。结果 TLC法鉴别色谱特征斑点明显,HPLC法专属性强,无杂质干扰。结论该法简便准确,灵敏度高,通用性好,适用于白带丸中黄柏质量的监控。

TLC and HPLC monitoring of quality of Baidai Wan SHEN Yu-lan,LUAN Jie,DIND Qing

Objective To investigate the epidemiology by genotyping among isolates,in order to provide a scientific basis for the control of nosocomial infection.Methods In this study,during the month of September from 2005 to 2010,188 clinical S.maltophilia isolates were collected from 34 different graded hospitals which were the members of Anhui Center for Surveillance of Bacterial Resistance.The isolates were identified by using the Microscan Walkaway-40 System.Total DNA of the 102 clinical isolates from 2005 to 2008 collection was extracted by suspending several overnight colonies in 0.5mL of double-distilled water and heating the mixture at 100℃ for 10 min.The epidemic spread of S.maltophilia isolates was detected by random primer PCR method for genotyping.Results By genotyping of the 188 isolates,we found that all the isolates in 2005,2007 and 2009 showed different geneotypes,but isolate 678 and 702 in 2006,isolate 824 and 826 in 2008,isolate 186 and 187 in 2010 shared the same geneotypes respectively.After tracking clinical data we found that they were from the same department in the same hospital respectively.Our results revealed that cross-colonization might be possible among the patients who are followed up at the same center.Conclusions The occurrence of the same geneotype isolates in the same department suggests that the bacteria have the risk of causing nosocomial infection epidemic.Therefore,clinicians should be aware of the bacteria in control underlying debilitating diseases of patients,and take measures to reduce the infection of the bacteria.