载紫杉醇PLGA微球在Skov3卵巢癌荷瘤小鼠体内的治疗效果

目的   应用乳酸-羟基乙酸共聚物(PLGA)制备载紫杉醇(PTX)PLGA微球，评价PTX-PLGA微球对荷瘤小鼠治疗效果。方法   采用4周龄雌性BALB/c-nu裸小鼠建模，将卵巢癌Skov3细胞株注射于裸鼠背部一侧，制备裸小鼠皮下移植瘤。设空白对照组、生理盐水组、PLGA微球悬液组、PTX注射液组和PTX-PLGA微球悬液组。分别对成瘤后荷瘤鼠进行治疗、观察记录、绘制肿瘤生长曲线。对Skov3移植瘤裸小鼠瘤内直接注射药物并应用免疫组化法检测移植瘤微血管密度(MVD)、相关死亡促进因子(Bad)。结果   给药6周后，PTX-PLGA微球组瘤内注射抑瘤效果明显大于其他各组(P＜0.05)，肿瘤体积明显减小，癌细胞增殖受到抑制。结论   PTX-PLGA微球瘤内直接注射对卵巢癌的治疗有明显体内抑瘤效果。

Treatment effect of paclitaxel loaded microspheres in Skov3 tumor-bearing nude mice

Objective   To prepare PLGA ［poly (lactic-co-glycolic acid)］ as the sustained-releasing microspheres loaded with paclitaxel and to evaluate the treatment effect on the implanted tumor induced by Skov3 cells in the nude mice. Methods   Four-week-old female BALB/c-nu mice were used as tumor models by injecting Skov3 cells at the back side, and they were evenly divided into four groups. And inner tumor injection was done by using physiological saline, the PLGA microspheres, the paclitaxel and the paclitaxel loaded PLGA microspheres separately. Then the mice were treated and observed and the tumor growth curves were recorded.Direct inner tumor injection was done and the MVD and Bad was measured by immunohistochemistry. Results   After 6 weeks of treatment, tumor size and volume of tumor models treated with PLGA sustained-releasing microspheres loaded with paclitaxel were significantly decreased, compared with those of other groups(P＜0.05). Conclusion   PLGA sustained-releasing microspheres loaded with paclitaxel by direct inner tumor injection has a remarkably inhibitory effect on ovarian cancer.