碘酸钾和双酚A对甲状腺乳头状癌细胞增殖的影响

目的   观察碘酸钾和双酚A对人甲状腺乳头状癌细胞BHP10-3增殖的影响。方法   采用10-3~10-10mol/L碘酸钾和10-3~10-10mol/L双酚A(BPA)，10-7mol/L碘酸钾+10-8mol/L双酚A培养BHP10-3细胞后，采用CCK-8细胞增殖活性法测定细胞增殖，采用流式细胞仪分析甲状腺乳头状癌细胞周期，采用western-blot印迹法和RT-PCR法分别检测细胞增殖周期调控基因（Cyclin D1）在蛋白和mRNA水平的表达。结果   与对照组细胞相比，10-7mol/L碘酸钾组、10-8mol/L双酚A组以及10-7mol/L碘酸钾联合10-8mol/L双酚A组，细胞吸光光度值增加（P<0.05）; G0-G1期细胞比例减少,  S期细胞比例增加(P<0.05)；CyclinD1蛋白和mRNA的表达量均增加（P<0.05）。结论   碘酸钾和双酚A在一定浓度范围内可以促进甲状腺乳头状癌细胞的增殖。

Effects of potassium iodate and bisphenol A on the proliferation of thyroid carcinoma cells

Objective   To observe the effects of potassium iodate and bisphenol A(BPA)on proliferation of thyroid carcinoma cells BHP10-3. Methods   10-3~10-10mol/L potassium iodate,  10-3~10-10mol/L bisphenol A and 10-7mol/L potassium iodate +10-8mol/L bisphenol A were added to the culture of BHP10-3 cells. Cell proliferation activity was examined by CCK-8 colorimetric assays,  and the cell cycles of the stimulated cells were analyzed by flow cytometer. The expression of Cyclin D1 protein was observed by western-blot,  and the expression of cyclinD1 mRNA was observed by RT-PCR. Results   Compared with the control group,  the absorbance of the potassium iodate group,  the BPA group and the potassium iodate+BPA group was higher (P<0.05), the proportion of cells in the G0-G1 phase decreased and proportion of cells in the S phase increased significantly (P<0.05), the expression levels of Cyclin D1 protein and mRNA were up-regulated significantly(P<0.05). Conclusion   The potassium iodate and BPA can promote the proliferation of thyroid papillary carcinoma cells in a certain concentration range.