Reactive oxidative species enhance amyloid toxicity in APP/PS1 mouse neurons |
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| Authors: | Bin Yang Xiaqin Sun Hilal Lashuel Yan Zhang |
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| Institution: | Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China 2 Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental Science, Minzu University of China, Beijing 100081, China 3 School of Life Sciences, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland |
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| Abstract: | Objective To investigate whether intracellular amyloid β (iAβ) induces toxicity in wild type (WT) and APP/PS1 mice, a mouse model of Alzheimer’s disease. Methods Different forms of Aβ aggregates were microinjected into cultured WT or APP/PS1 mouse hippocampal neurons. TUNEL staining was performed to examine neuronal cell death. Reactive oxidative species (ROS) were measured by MitoSOXTM Red mitochondrial superoxide indicator. Results Crude, monomer and protofibrilAβ induced more toxicity inAPP/PS1 neurons than in WT neurons. ROS are involved in mediating the vulnerability of APP/PS1 neurons to iAβ toxicity. Conclusion Oxidative stress may mediate cell death induced by iAβ in neurons. |
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| Keywords: | amyloid reactive oxidative species APP/PS1 aggregation toxicity |
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