| 中药材分子鉴别现场运用的策略与实践 |
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| 引用本文： | 袁媛,蒋超,黄璐琦.中药材分子鉴别现场运用的策略与实践[J].中国中药杂志,2013,38(16):2553-2555. |
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| 作者姓名： | 袁媛 蒋超 黄璐琦 |
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| 作者单位： | 中国中医科学院 中药资源中心, 北京 100700;中国中医科学院 中药资源中心, 北京 100700;中国中医科学院 中药资源中心, 北京 100700 |
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| 基金项目： | 北京市科技专项"道地中药材功能基因组研究北京市重点实验室2012年阶梯计划项目" |
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| 摘 要： | 在中药材实际生产与贸易交流中,中药材准确、快速鉴别一直是比较困难的工作。分子鉴别被认为是传统鉴别技术的有益补充,但目前分子鉴别方法仅止于实验室阶段,难以实现在产地、药市、药房等进行现场鉴别,极大的限制了其使用的范围和推广程度。其中,中药材DNA快速提取技术和DNA标记的快速检测技术是实现分子鉴别现场运用的两大瓶颈和关键问题。该研究开发了一套中药材分子鉴别现场运用模块系统,包括药材DNA快速提取模块、DNA标记检测模块和保障模块,并配备了相应的自主开发试剂盒、仪器装备。运用这套系统对金银花进行现场鉴别,可在40~60 min完成,且仪器装置简单、易于操作,为传统中药现场鉴别手段的有益补充。
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| 关 键 词： | 现场鉴别 分子鉴定 药材 |
| 收稿时间： | 2012/12/3 0:00:00 |
Strategy and practice on scene application of molecular identification in Chinese medicine materials |
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| YUAN Yuan,JIANG Chao and HUANG Lu-qi.Strategy and practice on scene application of molecular identification in Chinese medicine materials[J].China Journal of Chinese Materia Medica,2013,38(16):2553-2555. |
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| Authors: | YUAN Yuan JIANG Chao and HUANG Lu-qi |
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| Institution: | National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Accurate and fast identification of Chinese medicine materials has been more difficult work during production and trade. However the molecular identification at present was only used in the laboratory, and difficult to scene application in producing area, market and pharmacy that limited its usage greatly. There are two important technologic bottlenecks for scene application: DNA rapid extraction and fast detection of DNA markers. This study developed a module system for the scene application of molecular identification in Chinese medicine materials. |
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| Keywords: | scene identification molecular identification Chinese medicine materials |
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