长链非编码RNA SNHG16靶向微小RNA-7-5p对喉癌细胞增殖、迁移、侵袭的影响

目的 探讨长链非编码RNA SNHG16对喉癌细胞增殖、迁移、侵袭的影响及其作用机制.方法 选取2016年10月至2018年12月重庆医科大学附属第三医院收治的25例喉癌病人,于术中切取喉癌组织及其相应癌旁组织并冻存.体外培养喉癌Hep-2细胞,Hep-2细胞,按照随机数字表法分为si-NC组(转染无意义干扰序列si-...

The effect of SNHG16 regulate on proliferation, migration and invasion of laryngeal carcinoma cells by targeting miR-7-5p

Objective To investigate the effects of long non-coding RNA SNHG16 (LncRNA SNHG16) on proliferation, migration and invasion of laryngeal carcinoma cells and its mechanism. Methods Twenty-five patients with laryngeal cancer admitted to theThird Affiliated Hospital of Chongqing Medical University from October 2016 to December 2018 were selected, and the laryngeal can?cer tissue and its corresponding adjacent tissues were cut and frozen during the operation. Laryngeal carcinoma Hep-2 cells and Hep-2 cells were cultured in vitro, and they were randomly divided into si-NC group (transfected with meaningless interference sequence si-NC), si-SNHG16 group (transfected with si-SNHG16), and si-SNHG16+anti-miR-NC group (co-transfection si-SNHG16 and anti-miR-NC), si-SNHG16+anti-miR-7-5p group (co-transfection si-SNHG16 and anti-miR-7 -5p). The expression of SNHG16 and miR-7-5p in laryngeal carcinoma tissues was detected by qRT-PCR. MTT assay and Transwell assay were used to detect the changes of proliferation, migration and invasion of Hep-2 cells after inhibiting the expression of SNHG16. Bioinformatics was used to predict target miRNAs forSNHG16, and dual luciferase reporter experiments validated the targeting of SNHG16 and miR-7-5p.Inhibition of miR-7-5p expression verified the effect of SNHG16 on proliferation, migration and invasion of Hep-2 cells. Results The expression level of SNHG16 in la? ryngeal carcinoma was significantly increased (P<0.05), and the expression level of miR-7-5p was significantly decreased (P<0.05). Compared with the si-NC group, the inhibition of SNHG16 expression in the si-SNHG16 group significantly inhibited the proliferation of Hep-2 cells [48 h: (0.95±0.09) vs. (0.47±0.05),P < 0.05], and the number of migration [(124±12) vs. (51±5),P < 0.05] and invasion cells decreased significantly [(115±11) vs. (43±4),P < 0.05]. SNHG16 could negatively regulate the expression of miR-7-5p. Inhibition of miR-7-5p reversed the inhibitory effect of SNHG16 on proliferation, migration and invasion of Hep-2 cells. Conclusion Inhibition of SNHG16 expression negatively regulates the expression of miR-7-5p and attenuates the proliferation, migration and invasion ability of laryngeal carcinoma cells.