人参皂苷Rg3对胰腺癌裸鼠皮下移植瘤新生血管的影响

目的探究人参皂苷Rg3对胰腺癌裸鼠皮下移植瘤新生血管形成的影响。方法裸鼠背侧近右后肢处sc胰腺癌SW-1900细胞建立胰腺癌皮下移植瘤模型,将荷瘤裸鼠随机分为对照组及人参皂苷Rg3低、高剂量(10、20 mg/kg)组。给药组ip人参皂苷Rg3,对照组ip0.9%氯化钠溶液,每2天1次,连续4周。给药结束3d后裸鼠脱颈椎处死,取瘤组织。采用免疫组化法检测各组裸鼠瘤组织中内皮细胞标记物CD31表达,计算微血管密度;采用qRT-PCR和Westernblotting法检测各组裸鼠瘤组织中血管内皮钙黏素(vascularendothelialcadherin,VE-cadherin)、络氨酸激酶受体A2(erythropoietin-producing hepatocellular receptor A2,EphA2)m RNA和蛋白表达水平。结果与对照组比较,人参皂苷Rg3组胰腺癌裸鼠皮下移植瘤质量显著降低(P<0.05),微血管密度显著降低(P<0.05),VE-cadherin、EphA2m RNA和蛋白表达水平均显著降低(P<0.05)。结论人参皂苷Rg3可能通过调控VE-cadherin、EphA2m RNA和蛋白表达,从而抑制胰腺癌裸鼠皮下移植瘤新生血管形成。

Effect of ginsenoside Rg3 on neovascularization of subcutaneous transplantation tumor of pancreatic cancer in nude mice

Objective To explore the effect of ginsenoside Rg₃ on the angiogenesis of subcutaneously transplanted tumor of pancreatic cancer in nude mice.Methods Back of nude mice near the right hind limb were sc pancreatic cancer SW-1900 cells to establish a pancreatic cancer subcutaneous transplantation tumor model. Tumor-bearing nude mice were randomly divided into control group, low- and high-dose ginsenoside Rg₃ (10, 20 mg/kg) groups. Rats in administration group were ip ginsenoside Rg₃ and rats in control group were ip 0.9% sodium chloride solution, once every 2 d for four consecutive weeks. Three days after administration, nude mice were sacrificed by cervical vertebrae, and tumor tissues were collected. Immunohistochemical method was used to detect the expression of endothelial cell marker CD31 in the tumor tissues of nude mice, and the microvessel density was calculated. qRT-PCR and Western blotting were used to detect vascular endothelial cadherin (VE-cadherin), erythropoietin-producing hepatocellular receptor A2 (EphA2) mRNA and protein expression levels. Results Compared with the control group, the weight of subcutaneously transplanted pancreatic cancer nude mice in ginsenoside Rg₃ group were significantly reduced (P < 0.05); Microvessel density were significantly reduced (P < 0.05), mRNA and protein expression levels of VE-cadherin and EphA2 were significantly reduced (P < 0.05). Conclusion Ginsenoside Rg₃ can regulate the expression of VE-cadherin and EphA2 mRNA and protein expression, thereby inhibiting the angiogenesis of subcutaneously transplanted pancreatic cancer in nude mice.