逍遥散抗抑郁代谢特征综合分析及其调节能量代谢和神经递质机制研究

目的通过对多种生物样本中内源性代谢物特征进行综合分析,阐释逍遥散抗抑郁的作用机制。方法对慢性不可预知温和刺激(chronic unpredicted mild stress,CUMS)抑郁大鼠模型的血液、尿液、海马、肝脏、盲肠、粪便和临床患者的血浆、尿液等生物样本中逍遥散具有显著调节作用的差异代谢物进行代谢特征综合分析,归纳总结逍遥散在不同生物样本中调节的关键差异代谢物和代谢通路。结果逍遥散在不同生物样本中调节的相同差异代谢物有34个,其中11个变化趋势一致,涉及的关键代谢通路为能量代谢和神经递质通路等。结论逍遥散能够通过调节体内代谢平衡所在的代谢通路发挥抗抑郁作用,为系统挖掘逍遥散抗抑郁代谢机制提供依据。

Comprehensive analysis of antidepressant metabolic characteristics of Xiaoyao San and its mechanism of regulating energy metabolism and neurotransmitter

Objective To elucidate the antidepressant mechanism of Xiaoyao San(逍遥散) through a comprehensive analysis of the characteristics of endogenous metabolites in various biological samples. Methods The metabolic characteristics of different metabolites with significant regulating effects in biological samples(blood, urine, hippocampus, liver, cecum, feces) of chronic unpredicted mild stress(CUMS) rats model, plasma and urine of clinical patients were comprehensively analyzed. The key different metabolites and metabolic pathways regulated by Xiaoyao San in differential biological samples were summarized. Results Among different samples, Xiaoyao San regulated 34 different metabolites, 11 of which had the same change trend. The key metabolic pathways regulated by Xiaoyao San including energy metabolism and neurotransmitter pathways. Conclusion Xiaoyao San plays an antidepressant role by regulating the metabolic pathway of metabolic balance in vivo, which provides basis for systematically exploring the antidepressant metabolic mechanism of Xiaoyao San.