ODN2088减轻线粒体DNA致脓毒症大鼠急性肺损伤

目的:探讨血浆线粒体DNA(mtDNA)含量与脓毒症大鼠急性肺损伤(ALI)程度的关系及选择性TLR9受体抑制剂ODN2088对脓毒症大鼠ALI的保护作用.方法:按随机数表法将80只大鼠分为假手术(sham)组、脓毒症(sepsis)组、脓毒症大鼠腹腔注射mtDNA 1.4 mg/kg(sepsis+mtDNA)组、脓...

Relief of ODN2088 for Acute Lung Injury in Rats with Sepsis Induced by Mitochondrial DNA

Objective: To probe into the correlation between plasma mitochondrial DNA (mtDNA) content and severity of acute lung injury (ALI) in rats with sepsis and the protective effects of selective Toll-like receptor 9 (TLR9) inhibitor ODN2088 on ALI in rats with sepsis. Methods: According to the random number table method, 80 rats were divided into the sham surgery (sham) group, sepsis (sepsis) group, intraperitoneal injection of mtDNA 1.4 mg/kg (sepsis+mtDNA) group and intraperitoneal injection of mtDNA 1.4 mg/kg+ODN2088 1 mg/kg (sepsis+mtDNA+ODN) group, with 20 rats in each group. The model of sepsis complicated with ALI was established by cecal ligation and perforation (CLP). After surgery of 6 and 12 h, blood samples, bronchoalveolar lavage fluid (BALF) and lung tissues were collected to detect plasma mtDNA and TLR9/p38 mitogen activated protein kinase (MAPK) expression levels in lung tissues. Results: Compared with the sham group, the sepsis group had higher mortality, increased levels of plasma mtDNA and TLR9/p38 MAPK expression in lung tissues, and increased ALI degree lung injury score, inflammatory cells in BALF, lung (wet-dry)/dry weight ratio and gene expression, P<0.05]. Compared with the sepsis group, the mortality rate, plasma mtDNA, TLR9/p38MAPK expression and ALI degree increased in the sepsis+mtDNA group (P<0.05). Compared with the sepsis+mtDNA+ODN group, the mortality rate, plasma mtDNA, TLR9/p38MAPK expression and ALI degree decreased in the sepsis+mtDNA+ODN group (P<0.05). Conclusion: Plasma mtDNA is correlated with ALI induced by sepsis, and TLR9 inhibitor ODN2088 can relieve ALI in rats with sepsis induced by mtDNA.