经颅交流电刺激对缺血性脑卒中大鼠rs-fMRI的影响

目的:建立缺血性脑卒中大鼠右侧大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)模型,观察经颅交流电刺激(transcranial alternating current stimulation,tACS)干预对缺血性脑卒中大鼠行为学及静息态功能性磁共振成像(resting-s...

Effects of transcranial alternating current stimulation on resting-state functional magnetic resonance of MCAO rats

Objective:A stroke rat model(MCAO)was established to dynamically observe the effects of transcranial alternating current stimulation(tACS)intervention on behavioral performance and resting state functional magnetic resonance imaging(rs-fMRI)in cerebral ischemia rats.Method:Twenty-four male SD rats were randomly divided into sham-operation group(S group),model group(M group)and tACS group(T group).Body weight at five time points,neurological deficit scores(NDS)at six time points and rs-fMRI before modeling,at D1 and D10 were recorded,The effects of tACS on the brain function of MCAO rats were observed.Result:NDS in M group and T group were increased compared with S group at 2h,D1,D3,D5 and D7(P<0.05).T group were decreased when compared with M group at 3D,5D,7D(P<0.05).Body Weight in M group and T group at D1 decreased significantly compared with other time points(P<0.05);S group at D5 and D7 increased significantly compared with other time points(P<0.05);S group at D3 increased significantly compared with B group at D1(P<0.05);M group at D1,D3,D5,D7 decreased significantly compared with S group(P<0.05).Brain Imageing:At D10,the ReHo in T group increased significantly compared with S group in left prefrontal cortex,right prefrontal cortex and left anterior cingulate cortex;the ReHo at D10 were increased significantly compared with that at D1 in T group in right prefrontal cortex,right hippocampus(P<0.05).At D1,the voxel-based morphometry(VBM)in T group increased significantly compared with S group in left of olfactory bulb,right interpeduncular nucleus,left hypothalamic region.The VBM at D10 were decreased significantly compared with that at D1 in T group in right hippocampus,right brachium of the superior colliculus,right striatum;and the volume of gray matter at D10 in T group in whole brain was increased significantly compared with that in S group(P<0.05).At D1,the ALFF in T group increased significantly compared with that in S group in right/left glomerular layer of the olfactory bulb,striatum;At D10,the ALFF in T group increased significantly compared with that in S group in Cerebellum,striatum,prefrontal cortex and olfactory bulb(P<0.05).Conclusion:The early intervention of tACS had effects on the brain function of MCAO rats,which may related to the enhancement of neuronal activity and the increase of gray matter volume.In addition to learning and memory-related brain regions,tACS may also affect the motor-related brain regions and promote motor rehabilitation.