大黄酸对小鼠非酒精性脂肪肝病的治疗效果分析

目的:探讨脂肪相关基因(fat mass and obesity associated gene,FTO)及其抑制剂大黄酸(rhein)在非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)中的作用.方法:采用高脂饲料喂养的方法构建NAFLD小鼠模型,利用灌胃的方式使用rh...

Therapentic effect of rhein on non-alcoholic fatty liver disease in mice

[Abstract] Objective: To investigate the role of fat mass and obesity associated gene (FTO) and its inhibitor Rhein in the development of nonalcoholic fatty liver disease (NAFLD). Methods: A NAFLD mouse model was constructed by high-fat diet and treated with Rhein by gavage. In order to evaluate the success of modeling and the effect of treatment, HE staining and oil red staining were used to determine the fat deposition in mouse liver tissues, and Western blot was used to detect the changes of FTO protein expression level in mouse liver tissues. Results: The results of HE staining and oil red staining showed that Rhein could reduce fatty deposition in liver tissues of NAFLD mice, promote liver functional recovery, and reduce FTO protein expression in liver tissues. Conclusions: Rhein can reduce the expression of FTO protein in liver tissue of NAFLD mouse model and has the potential to treat NAFLD.