基于网络药理学和设计空间的乳腺康提取工艺研究

目的 通过网络药理学及设计空间法对乳腺康提取工艺参数进行研究.方法 借助网络药理学对乳腺康潜在的活性成分进行筛选,并与酪氨酸激酶进行分子对接,结合《中国药典》2020年版确定指标性成分,采用HPLC法运用设计空间进行乳腺康提取工艺研究.结果 筛选出乳腺康中甘草查尔酮A、川陈皮素、蒲公英甾醇等核心成分与酪氨酸激酶的亲和力...

Research on extraction process of Ruxiankang based on network pharmacology and design space

Objective To explore the extraction process parameters of Ruxiankang(乳腺康)through network pharmacology and design space.Methods With the help of network pharmacology,the active ingredients of Ruxiankang were screened,and molecular docking with tyrosine kinase was carried out,combined withChinese Pharmacopoeia2020 edition to determine the index components,and the extraction process of Ruxiankang by high performance liquid chromatography and design space was studied.Results The core components of Ruxiankang screened,such as licochalcone A,nobiletin,and taraxasterol,had affinity with tyrosine kinases similar to those recommended in the clinic,and the optimal extraction process was obtained in the design space:the immersion time was 30 min,and the solvent volume was 12 times,extraction time was 45—75 min,ethanol concentration was 65%—80%,for 2—3 extraction times.Conclusion The process obtained in the experiment is reasonable and feasible,the verification experiment is close to the predicted value of the theoretical value,which has certain practical value.The Ruxiankang extraction process is based on the QbD concept,which is stable and reliable,and provides ideas for its further process development and quality control.