神经纤毛蛋白 1和血管内皮生长因子受体 2在肝细胞癌组织中的表达和临床意义

目的 检测神经纤毛蛋白1(NRP-1)和血管内皮生长因子受体2(VEGFR2)在肝细胞癌组织及其癌旁正常肝组织中的表达情况,同时探讨NRP-1和VEGFR2的表达与肝细胞癌病人预后的关系.方法 收集2014年11月至2018年11月在徐州医科大学附属医院肝胆外科行手术切除并经病理学确诊为肝细胞癌病人的肿瘤石蜡标本40例及其癌旁正常肝组织标本20例,应用免疫组织化学法检测肝细胞癌组织及正常肝组织中NRP-1和VEGFR2的表达情况.对病人进行至少为期1年的随访,末次随访时间为2019年11月.结果 NRP-1、VEGFR2在肝细胞癌组织中的表达[(0.398±0.051)、(0.454±0.058)]明显高于正常肝组织[(0.244±0.095)、(0.337±0.064)](均P<0.05).NRP-1、VEGFR2表达与肝细胞癌病人的肿瘤最大径、有无血管侵犯、分化程度、TNM分期明显相关(均P<0.05),而与病人的性别、年龄、有无慢性肝炎病毒感染、甲胎蛋白水平、肿瘤数目、有无肝硬化、Child-Pugh分级无关(均P>0.05).NRP-1和与VEGFR2的表达呈正相关(r=0.789,P<0.001).此外,NRP-1、VEGFR2表达高的肝细胞癌病人的术后1年内复发率高[63.64％(14/22)、55.00％(11/20)],而NRP-1、VEGFR2表达低的肝细胞癌病人的术后1年复发率较低[5.56％(1/18)、20.00％(4/20)](均P<0.05).结论 NRP-1、VEGFR2在肝细胞癌组织中均有高表达,在肝细胞癌的发生和发展中起重要作用,而NRP-1、VEGFR2的高表达提示病人预后不良.

Expressions and clinical significance of NRP-1and VEGFR2 in hepatocellular carcinoma

Objective To detect the expression of neuropilin 1 (NRP-1) and vascular endothelial growth factor receptor 2 (VEGFR2)in hepatocellular carcinoma (HCC) tissues and normal liver tissues adjacent to the cancer, and explore the relationship between the ex?pressions of NRP-1 and VEGFR2 with the prognosis of HCC patients.Methods The data of 40 HCC patients who underwent completeresection and pathological diagnosis from November 2014 to November 2018 in The Affiliated Hospital of Xuzhou Medical Universitywere collected. The expression of NRP-1 and VEGFR2 in 40 HCC tissues and 20 paracancerous liver tissues was detected by immuno?histochemistry. All patients were followed up after operation, the last followed-up time was November 2019.Results The expression rate of NRP-1 and VEGFR2 in HCC tissues [(0.398±0.051), (0.454±0.058)] was significantly higher than that in paracancerous normalliver tissues [(0.244±0.095), (0.337±0.064)] (all P<0.05). The expression of NRP-1 and VEGFR2 was significantly correlated with themaximum diameter, vascular invasion, differentiation and TNM stage of HCC patients(all P<0.05). There was no significant relationshipbetween sex, age, chronic hepatitis virus infection, alpha fetoprotein level, tumor number,liver cirrhosis and Child-Pugh grade(all P> 0.05).There was a positive correlation between the expression of NRP-1 and VEGFR2(r=0.789,P<0.001).In addition, the recurrence rate within 1 year after operation was higher in HCC patients with high expression of NRP-1 and VEGFR2 [63.64%(14/22), 55.00%(11/ 20)], while the recurrence rate was lower in patients with low expression of NRP-1 and VEGFR2 in HCC patients [5.56%(1/18), 20.00% (4/20)] (all P<0.05).Conclusion NRP-1 and VEGFR2 are highly expressed in HCC tissues, and may play an important role in the oc?currence and development of HCC, while the high expression of NRP-1 and VEGFR2 indicates poor prognosis.